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Journal of Bacteriology, October 1999, p. 6124-6132, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Phylogenetic Analysis of L4-Mediated Autogenous Control of the S10 Ribosomal Protein Operon

Todd Allen,1 Ping Shen,2 Leigh Samsel,1 Raymond Liu,1 Lasse Lindahl,1,2 and Janice M. Zengel1,2,*

Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, Maryland 21250,1 and Department of Biology, University of Rochester, Rochester, New York 146272

Received 12 March 1999/Accepted 25 July 1999

We investigated the regulation of the S10 ribosomal protein (r-protein) operon among members of the gamma subdivision of the proteobacteria, which includes Escherichia coli. In E. coli, this 11-gene operon is autogenously controlled by r-protein L4. This regulation requires specific determinants within the untranslated leader of the mRNA. Secondary structure analysis of the S10 leaders of five enterobacteria (Salmonella typhimurium, Citrobacter freundii, Yersinia enterocolitica, Serratia marcescens, and Morganella morganii) and two nonenteric members of the gamma subdivision (Haemophilus influenzae and Vibrio cholerae) shows that these foreign leaders share significant structural homology with the E. coli leader, particularly in the region which is critical for L4-mediated autogenous control in E. coli. Moreover, these heterologous leaders produce a regulatory response to L4 oversynthesis in E. coli. Our results suggest that an E. coli-like L4-mediated regulatory mechanism may operate in all of these species. However, the mechanism is not universally conserved among the gamma subdivision members, since at least one, Pseudomonas aeruginosa, does not contain the required S10 leader features, and its leader cannot provide the signals for regulation by L4 in E. coli. We speculate that L4-mediated autogenous control developed during the evolution of the gamma branch of proteobacteria.

* Corresponding author. Mailing address: Department of Biological Sciences, UMBC, 1000 Hilltop Circle, Baltimore, MD 21250. Phone: (410) 455-2876. Fax: (410) 455-3875. E-mail: zengel{at}umbc.edu.

Journal of Bacteriology, October 1999, p. 6124-6132, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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