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Journal of Bacteriology, October 1999, p. 6188-6191, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Analysis of the Corynebacterium glutamicum dapA Promoter

Pavla Vasicová,1 Miroslav Pátek,1,* Jan Nesvera,1 Hermann Sahm,2 and Bernhard Eikmanns2,dagger

Institute of Microbiology, Academy of Sciences of the Czech Republic, CZ-14220 Praha 4, Czech Republic,1 and Institute of Biotechnology, Forschungszentrum Jülich, D-52425 Jülich, Germany2

Received 26 April 1999/Accepted 29 July 1999

Deletion and mutational analysis of the promoter P-dapA from Corynebacterium glutamicum was performed to identify regions and particular nucleotides important for its function. An extended -10 region and a stretch of six T's at positions -55 to -50 were found to be the most important elements in the promoter function. The results of mutational analysis of P-dapA are consistent with the conclusions of statistical computer-aided analysis of 44 C. glutamicum promoter sequences.

* Corresponding author. Mailing address: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, CZ-14220 Praha 4, Czech Republic. Phone: (4202) 4752354. Fax: (4202) 4722257. E-mail: patek{at}biomed.cas.cz.

dagger Present address: University of Ulm, Department of Applied Microbiology, D-89069 Ulm, Germany.

Journal of Bacteriology, October 1999, p. 6188-6191, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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