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Journal of Bacteriology, October 1999, p. 6200-6204, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Purification and Characterization of a Novel Naphthalene Dioxygenase from Rhodococcus sp. Strain NCIMB12038

Michael J. Larkin,* Christopher C. R. Allen, Leonid A. Kulakov, and David A. Lipscomb

The Questor Centre, The Queen's University of Belfast, Belfast BT9 5AG, and School of Biology and Biochemistry, Medical Biology Centre, The Queen's University of Belfast, Belfast BT9 7BL, Northern Ireland

Received 1 March 1999/Accepted 5 July 1999

We report here the characterization of the catalytic component (ISPNAR) of a new naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038. The genes encoding the two subunits of ISPNAR are not homologous to their previously characterized counterparts in Pseudomonas. The deduced amino acid sequences have only 33 and 29% identity with the corresponding subunits in Pseudomonas putida NCIB 9816-4, for which the tertiary structure has been reported.

* Corresponding author. Mailing address: The Questor Centre, The Queen's University of Belfast, The David Keir Building, Stranmillis Road, Belfast BT9 5AG, Northern Ireland. Phone: 44 1232 335577. Fax: 44 1232 661462. E-mail: M.Larkin{at}qub.ac.uk.

Journal of Bacteriology, October 1999, p. 6200-6204, Vol. 181, No. 19
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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