This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gribaldo, S.
Right arrow Articles by Cammarano, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gribaldo, S.
Right arrow Articles by Cammarano, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 1999, p. 434-443, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Discontinuous Occurrence of the hsp70 (dnaK) Gene among Archaea and Sequence Features of HSP70 Suggest a Novel Outlook on Phylogenies Inferred from This Protein

Simonetta Gribaldo,1 Valentina Lumia,1 Roberta Creti,1 Everly Conway de Macario,2 Annamaria Sanangelantoni,3 and Piero Cammarano1,*

Istituto Pasteur Fondazione Cenci-Bolognetti, Dipartimento Biotecnologie Cellulari ed Ematologia, Università di Roma I, Policlinico Umberto I°, 00161 Roma,1 and Dipartimento di Genetica e Microbiologia "A. Buzzati Traverso," Università di Pavia, 27100 Pavia,3 Italy, and Wadsworth Center, Division of Molecular Medicine, New York State Department of Health, and Department of Biomedical Sciences, School of Public Health, The University at Albany, Albany, New York 12201-05092

Received 6 July 1998/Accepted 19 October 1998

Occurrence of the hsp70 (dnaK) gene was investigated in various members of the domain Archaea comprising both euryarchaeotes and crenarchaeotes and in the hyperthermophilic bacteria Aquifex pyrophilus and Thermotoga maritima representing the deepest offshoots in phylogenetic trees of bacterial 16S rRNA sequences. The gene was not detected in 8 of 10 archaea examined but was found in A. pyrophilus and T. maritima, from which it was cloned and sequenced. Comparative analyses of the HSP70 amino acid sequences encoded in these genes, and others in the databases, showed that (i) in accordance with the vicinities seen in rRNA-based trees, the proteins from A. pyrophilus and T. maritima form a thermophilic cluster with that from the green nonsulfur bacterium Thermomicrobium roseum and are unrelated to their counterparts from gram-positive bacteria, proteobacteria/mitochondria, chlamydiae/spirochetes, deinococci, and cyanobacteria/chloroplasts; (ii) the T. maritima HSP70 clusters with the homologues from the archaea Methanobacterium thermoautotrophicum and Thermoplasma acidophilum, in contrast to the postulated unique kinship between archaea and gram-positive bacteria; and (iii) there are exceptions to the reported association between an insert in HSP70 and gram negativity, or vice versa, absence of insert and gram positivity. Notably, the HSP70 from T. maritima lacks the insert, although T. maritima is phylogenetically unrelated to the gram-positive bacteria. These results, along with the absence of hsp70 (dnaK) in various archaea and its presence in others, suggest that (i) different taxa retained either one or the other of two hsp70 (dnaK) versions (with or without insert), regardless of phylogenetic position; and (ii) archaea are aboriginally devoid of hsp70 (dnaK), and those that have it must have received it from phylogenetically diverse bacteria via lateral gene transfer events that did not involve replacement of an endogenous hsp70 (dnaK) gene.

* Corresponding author. Mailing address: Dipt. Biotecnologie Cellulari, Sezione Genetica Molecolare, Policlinico Umberto I°, Viale Regina Elena 324, 00161, Roma, Italy. Phone: 0039-0-6-4940609. Fax: 0039-0-6-4462891. E-mail: Cammarano{at}bce.med.uniroma1.it.

Journal of Bacteriology, January 1999, p. 434-443, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Karlin, S., Mrazek, J., Ma, J., Brocchieri, L. (2005). Predicted highly expressed genes in archaeal genomes. Proc. Natl. Acad. Sci. USA 102: 7303-7308 [Abstract] [Full Text]  
  • Goulhen, F., Grenier, D., Mayrand, D. (2003). ORAL MICROBIAL HEAT-SHOCK PROTEINS AND THEIR POTENTIAL CONTRIBUTIONS TO INFECTIONS. CROBM 14: 399-412 [Abstract] [Full Text]  
  • Klunker, D., Haas, B., Hirtreiter, A., Figueiredo, L., Naylor, D. J., Pfeifer, G., Muller, V., Deppenmeier, U., Gottschalk, G., Hartl, F. U., Hayer-Hartl, M. (2003). Coexistence of Group I and Group II Chaperonins in the Archaeon Methanosarcina mazei. J. Biol. Chem. 278: 33256-33267 [Abstract] [Full Text]  
  • Daubin, V., Gouy, M., Perriere, G. (2002). A Phylogenomic Approach to Bacterial Phylogeny: Evidence of a Core of Genes Sharing a Common History. Genome Res 12: 1080-1090 [Abstract] [Full Text]  
  • Narberhaus, F. (2002). {alpha}-Crystallin-Type Heat Shock Proteins: Socializing Minichaperones in the Context of a Multichaperone Network. Microbiol. Mol. Biol. Rev. 66: 64-93 [Abstract] [Full Text]  
  • Nesb, C. L., L'Haridon, S., Stetter, K. O., Doolittle, W. F. (2001). Phylogenetic Analyses of Two "Archaeal" Genes in Thermotoga maritima Reveal Multiple Transfers Between Archaea and Bacteria. Mol Biol Evol 18: 362-375 [Abstract] [Full Text]  
  • Macario, A. J. L., Lange, M., Ahring, B. K., De Macario, E. C. (1999). Stress Genes and Proteins in the Archaea. Microbiol. Mol. Biol. Rev. 63: 923-967 [Abstract] [Full Text]  
  • Macario, A. J. L., de Macario, E. C. (1999). The Archaeal Molecular Chaperone Machine: Peculiarities and Paradoxes. Genetics 152: 1277-1283 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»