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Journal of Bacteriology, January 1999, p. 610-617, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Polypeptide with Similarity to Phycocyanin alpha -Subunit Phycocyanobilin Lyase Involved in Degradation of Phycobilisomesdagger ,Dagger

Nadia Dolganov and Arthur R. Grossman*

Department of Plant Biology, The Carnegie Institution of Washington, Stanford, California 94305

Received 30 July 1998/Accepted 12 October 1998

To optimize the utilization of photosynthate and avoid damage that can result from the absorption of excess excitation energy, photosynthetic organisms must rapidly modify the synthesis and activities of components of the photosynthetic apparatus in response to environmental cues. During nutrient-limited growth, cyanobacteria degrade their light-harvesting complex, the phycobilisome, and dramatically reduce the rate of photosynthetic electron transport. In this report, we describe the isolation and characterization of a cyanobacterial mutant that does not degrade its phycobilisomes during either sulfur or nitrogen limitation and exhibits an increased ratio of phycocyanin to chlorophyll during nutrient-replete growth. The mutant phenotype was complemented by a gene encoding a polypeptide with similarities to polypeptides that catalyze covalent bond formation between linear tetrapyrrole chromophores and subunits of apophycobiliproteins. The complementing gene, designated nblB, is expressed at approximately the same level in cells grown in nutrient-replete medium and medium devoid of either sulfur or nitrogen. These results suggest that the NblB polypeptide may be a constitutive part of the machinery that coordinates phycobilisome degradation with environmental conditions.

* Corresponding author. Mailing address: Department of Plant Biology, The Carnegie Institution of Washington, 260 Panama St., Stanford, CA 94305. Phone: (650) 325-1521, ext. 212. Fax: (650) 325-6857. E-mail: arthur{at}andrew.stanford.edu.

dagger We dedicate this paper to Wolfhart Ruediger in celebration of his 65th birthday. Wolfhart has devoted much of his scientific career to analyzing the structure and function of tetrapyrroles in photosynthetic organisms.

Dagger Carnegie Institution of Washington publication no. 1377.

Journal of Bacteriology, January 1999, p. 610-617, Vol. 181, No. 2
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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