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Journal of Bacteriology, October 1999, p. 6264-6270, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Second Operator Is Involved in Pseudomonas aeruginosa Elastase (lasB) Activation

Ronda M. Anderson, Chad A. Zimprich, and Lynn Rust*

Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, North Dakota 58105

Received 29 March 1999/Accepted 26 July 1999

Pseudomonas aeruginosa LasB elastase gene (lasB) transcription is controlled by the two-component quorum-sensing system of LasR, and the autoinducer, 3OC12-HSL (N-3-[oxododecanoyl]homoserine lactone). LasR and 3OC12-HSL-mediated lasB activation requires a functional operator sequence (OP1) in the lasB promoter region. Optimal activation of lasB, however, requires a second sequence of 70% identity to OP1, named OP2, located 43 bp upstream of OP1. In this study, we used sequence substitutions and insertion mutations in lasBp-lacZ fusion plasmids to explore the role of OP2 in lasB activation. Our results demonstrate that (i) OP1 and OP2 synergistically mediate lasB activation; (ii) OP2, like OP1, responds to LasR and 3OC12-HSL; and (iii) the putative autoinducer-binding domain of LasR is not required for synergistic activation from OP1 and OP2.

* Corresponding author. Mailing address: Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND 58105. Phone: (701) 231-7848. Fax: (701) 231-7514. E-mail: lyrust{at}prairie.nodak.edu.

Journal of Bacteriology, October 1999, p. 6264-6270, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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