This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hõrak, R.
Right arrow Articles by Kivisaar, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hõrak, R.
Right arrow Articles by Kivisaar, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 1999, p. 6312-6318, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Regulation of the Transposase of Tn4652 by the Transposon-Encoded Protein TnpC

Rita Hõrak* and Maia Kivisaar

Department of Genetics, Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, 51010 Tartu, Estonia

Received 17 May 1999/Accepted 11 August 1999

Transposition is a DNA reorganization reaction potentially deleterious for the host. The frequency of transposition is limited by the amount of transposase. Therefore, strict regulation of a transposase is required to keep control over the destructive multiplication of the mobile element. We have shown previously that the expression of the transposase (tnpA) of the Pseudomonas putida PaW85 transposon Tn4652 is positively affected by integration host factor. Here, we present evidence that the amount of the transposase of Tn4652 in P. putida cells is controlled by the transposon-encoded protein (TnpC). Sequence analysis of the 120-amino-acid-long TnpC, coded just downstream of the tnpA gene, showed that it has remarkable similarity to the putative polypeptide encoded by the mercury resistance transposon Tn5041. As determined by quantitative Western blot analysis, the abundance of TnpA was reduced up to 10-fold in the intact tnpC background. In vivo experiments using transcriptional and translational fusions of the tnpA gene and the reporter gene gusA indicated that TnpC operates in the regulation of the transposase of Tn4652 at the post-transcriptional level.

* Corresponding author. Mailing address: Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, 23 Riia Street, 51010 Tartu, Estonia. Phone: 372-7-375015. Fax: 372-7-420286. E-mail: rhorak{at}ebc.ee.

Journal of Bacteriology, October 1999, p. 6312-6318, Vol. 181, No. 20
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Teras, R., Jakovleva, J., Kivisaar, M. (2009). Fis negatively affects binding of Tn4652 transposase by out-competing IHF from the left end of Tn4652. Microbiology 155: 1203-1214 [Abstract] [Full Text]  
  • Miyakoshi, M., Shintani, M., Terabayashi, T., Kai, S., Yamane, H., Nojiri, H. (2007). Transcriptome Analysis of Pseudomonas putida KT2440 Harboring the Completely Sequenced IncP-7 Plasmid pCAR1. J. Bacteriol. 189: 6849-6860 [Abstract] [Full Text]  
  • Tuffin, I. M., de Groot, P., Deane, S. M., Rawlings, D. E. (2005). An unusual Tn21-like transposon containing an ars operon is present in highly arsenic-resistant strains of the biomining bacterium Acidithiobacillus caldus. Microbiology 151: 3027-3039 [Abstract] [Full Text]  
  • Tark, M., Tover, A., Tarassova, K., Tegova, R., Kivi, G., Horak, R., Kivisaar, M. (2005). A DNA Polymerase V Homologue Encoded by TOL Plasmid pWW0 Confers Evolutionary Fitness on Pseudomonas putida under Conditions of Environmental Stress. J. Bacteriol. 187: 5203-5213 [Abstract] [Full Text]  
  • Kholodii, G., Gorlenko, Zh., Mindlin, S., Hobman, J., Nikiforov, V. (2002). Tn5041-like transposons: molecular diversity, evolutionary relationships and distribution of distinct variants in environmental bacteria. Microbiology 148: 3569-3582 [Abstract] [Full Text]  
  • Genka, H., Nagata, Y., Tsuda, M. (2002). Site-Specific Recombination System Encoded by Toluene Catabolic Transposon Tn4651. J. Bacteriol. 184: 4757-4766 [Abstract] [Full Text]  
  • Ilves, H., Horak, R., Kivisaar, M. (2001). Involvement of {sigma}S in Starvation-Induced Transposition of Pseudomonas putida Transposon Tn4652. J. Bacteriol. 183: 5445-5448 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»