This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kita, K.
Right arrow Articles by Tanaka, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kita, K.
Right arrow Articles by Tanaka, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 1999, p. 6822-6827, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evidence of Horizontal Transfer of the EcoO109I Restriction-Modification Gene to Escherichia coli Chromosomal DNA

Keiko Kita,1,* Junko Tsuda,1 Toshinobu Kato,1 Kenji Okamoto,1 Hideshi Yanase,1 and Masashi Tanaka2

Department of Biotechnology, Tottori University, 4-101 Koyama, Tottori 680-8552,1 and Gifu International Institute of Biotechnology, Yagi Memorial Park, Mitake, Gifu 505-0116,2 Japan

Received 17 June 1999/Accepted 13 August 1999

A DNA fragment carrying the genes coding for EcoO109I endonuclease and EcoO109I methylase, which recognize the nucleotide sequence 5'-(A/G)GGNCC(C/T)-3', was cloned from the chromosomal DNA of Escherichia coli H709c. The EcoO109I restriction-modification (R-M) system was found to be inserted between the int and psu genes from satellite bacteriophage P4, which were lysogenized in the chromosome at the P4 phage attachment site of the corresponding leuX gene observed in E. coli K-12 chromosomal DNA. The sid gene of the prophage was inactivated by insertion of one copy of IS21. These findings may shed light on the horizontal transfer and stable maintenance of the R-M system.

* Corresponding author. Mailing address: Department of Biotechnology, Tottori University, 4-101 Koyama, Tottori 680-8552, Japan. Phone: 81-857-31-5277. Fax: 81-857-31-0881. E-mail: kita{at}bio.tottori-u.ac.jp.

Journal of Bacteriology, November 1999, p. 6822-6827, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ooka, T., Ogura, Y., Asadulghani, M., Ohnishi, M., Nakayama, K., Terajima, J., Watanabe, H., Hayashi, T. (2009). Inference of the impact of insertion sequence (IS) elements on bacterial genome diversification through analysis of small-size structural polymorphisms in Escherichia coli O157 genomes. Genome Res 19: 1809-1816 [Abstract] [Full Text]  
  • Steele, M., Ziebell, K., Zhang, Y., Benson, A., Johnson, R., Laing, C., Taboada, E., Gannon, V. (2009). Genomic Regions Conserved in Lineage II Escherichia coli O157:H7 Strains. Appl. Environ. Microbiol. 75: 3271-3280 [Abstract] [Full Text]  
  • Piazzolla, D., Cali, S., Spoldi, E., Forti, F., Sala, C., Magnoni, F., Deho, G., Ghisotti, D. (2006). Expression of phage P4 integrase is regulated negatively by both Int and Vis.. J. Gen. Virol. 87: 2423-2431 [Abstract] [Full Text]  
  • Kita, K., Kawakami, H., Tanaka, H. (2003). Evidence for Horizontal Transfer of the EcoT38I Restriction- Modification Gene to Chromosomal DNA by the P2 Phage and Diversity of Defective P2 Prophages in Escherichiacoli TH38 Strains. J. Bacteriol. 185: 2296-2305 [Abstract] [Full Text]  
  • Naderer, M., Brust, J. R., Knowle, D., Blumenthal, R. M. (2002). Mobility of a Restriction-Modification System Revealed by Its Genetic Contexts in Three Hosts. J. Bacteriol. 184: 2411-2419 [Abstract] [Full Text]  
  • Ohshima, H., Matsuoka, S., Asai, K., Sadaie, Y. (2002). Molecular Organization of Intrinsic Restriction and Modification Genes BsuM of Bacillus subtilis Marburg. J. Bacteriol. 184: 381-389 [Abstract] [Full Text]  
  • Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y. (2001). Distribution of the SsuDAT1I Restriction-Modification System among Different Serotypes of Streptococcus suis. J. Bacteriol. 183: 5436-5440 [Abstract] [Full Text]  
  • Kobayashi, I. (2001). Behavior of restriction-modification systems as selfish mobile elements and their impact on genome evolution. Nucleic Acids Res 29: 3742-3756 [Abstract] [Full Text]  
  • Sekizaki, T., Otani, Y., Osaki, M., Takamatsu, D., Shimoji, Y. (2001). Evidence for Horizontal Transfer of SsuDAT1I Restriction-Modification Genes to the Streptococcus suis Genome. J. Bacteriol. 183: 500-511 [Abstract] [Full Text]  
  • Xu, Q., Stickel, S., Roberts, R. J., Blaser, M. J., Morgan, R. D. (2000). Purification of the Novel Endonuclease, Hpy188I, and Cloning of Its Restriction-Modification Genes Reveal Evidence of Its Horizontal Transfer to the Helicobacter pylori Genome. J. Biol. Chem. 275: 17086-17093 [Abstract] [Full Text]  
  • Rocha, E. P.C., Danchin, A., Viari, A. (2001). Evolutionary Role of Restriction/Modification Systems as Revealed by Comparative Genome Analysis. Genome Res 11: 946-958 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»