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Journal of Bacteriology, November 1999, p. 6828-6831, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mutations in the Regulatory Gene hrpG of Xanthomonas campestris pv. vesicatoria Result in Constitutive Expression of All hrp Genes

Kai Wengelnik,dagger Ombeline Rossier,Dagger and Ulla Bonas*

Centre National de la Recherche Scientifique, Institut des Sciences Végétales, 91190 Gif-sur-Yvette, France

Received 20 May 1999/Accepted 3 July 1999

hrpG is a key regulatory gene for transcriptional activation of pathogenicity genes (hrp) of Xanthomonas campestris pv. vesicatoria. We identified three mutations in hrpG which render hrp gene expression constitutive in normally suppressing medium. The mutations in hrpG result in novel amino acid substitutions compared to mutations in related proteins, such as OmpR. In addition, mutated hrpG enhances the timing and intensity of plant reactions in infection assays.

* Corresponding author. Present address: Institut für Genetik, Martin-Luther-Universität, 06099 Halle, Germany. Phone: 49 345 55 26 290. Fax: 49 345 55 27 259. E-mail: bonas{at}genetik.uni-halle.de.

dagger Present address: Imperial College, Department of Biology, London SW7-2AZ, United Kingdom.

Dagger Present address: Institut für Genetik, Martin-Luther-Universität, 06099 Halle, Germany.

Journal of Bacteriology, November 1999, p. 6828-6831, Vol. 181, No. 21
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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