This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Seong Kim, J.
Right arrow Articles by Sun Yum, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seong Kim, J.
Right arrow Articles by Sun Yum, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 1999, p. 6969-6976, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Cloning and Characterization of the Helicobacter pylori fliD Gene, an Essential Factor in Flagellar Structure and Motility

Jang Seong Kim, Ji Hoon Chang, Soo Il Chung, and Jung Sun Yum*

Mogam Biotechnology Research Institute, Koosung-myon, Yongin-city, Kyonggi-do 449-910, Korea

Received 12 March 1999/Accepted 7 September 1999

Helicobacter pylori colonizes the human stomach and can cause gastroduodenal disease. Flagellar motility is regarded as a major factor in the colonizing ability of H. pylori. The functional roles of flagellar structural proteins other than FlaA, FlaB, and FlgE are not well understood. The fliD operon of H. pylori consists of flaG, fliD, and fliS genes, in the order stated, under the control of a sigma 28-dependent promoter. In an effort to elucidate the function of the FliD protein, a hook-associated protein 2 homologue, in flagellar morphogenesis and motility, the fliD gene (2,058 bp) was cloned and isogenic mutants were constructed by disruption of the fliD gene with a kanamycin resistance cassette and electroporation-mediated allelic-exchange mutagenesis. In the fliD mutant, morphologically abnormal flagellar appendages in which very little filament elongation was apparent were observed. The fliD mutant strain was completely nonmotile, indicating that these abnormal flagella were functionally defective. Furthermore, the isogenic fliD mutant of H. pylori SS1, a mouse-adapted strain, was not able to colonize the gastric mucosae of host mice. These results suggest that H. pylori FliD is an essential element in the assembly of the functional flagella that are required for colonization of the gastric mucosa.

* Corresponding author. Mailing address: Mogam Biotechnology Research Institute, 341 Pojung-ri, Koosung-myon, Yongin-city, Kyonggi-do 449-910, Korea. Phone: 82-331-262-3851. Fax: 82-331-262-6622. E-mail: jsyum{at}kgcc.co.kr.

Journal of Bacteriology, November 1999, p. 6969-6976, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Franco, A. T., Friedman, D. B., Nagy, T. A., Romero-Gallo, J., Krishna, U., Kendall, A., Israel, D. A., Tegtmeyer, N., Washington, M. K., Peek, R. M. Jr. (2009). Delineation of a Carcinogenic Helicobacter pylori Proteome. Mol. Cell. Proteomics 8: 1947-1958 [Abstract] [Full Text]  
  • Smith, T. G., Pereira, L., Hoover, T. R. (2009). Helicobacter pylori FlhB processing-deficient variants affect flagellar assembly but not flagellar gene expression. Microbiology 155: 1170-1180 [Abstract] [Full Text]  
  • Pereira, L., Hoover, T. R. (2005). Stable Accumulation of {sigma}54 in Helicobacter pylori Requires the Novel Protein HP0958. J. Bacteriol. 187: 4463-4469 [Abstract] [Full Text]  
  • Stintzi, A., Marlow, D., Palyada, K., Naikare, H., Panciera, R., Whitworth, L., Clarke, C. (2005). Use of Genome-Wide Expression Profiling and Mutagenesis To Study the Intestinal Lifestyle of Campylobacter jejuni. Infect. Immun. 73: 1797-1810 [Abstract] [Full Text]  
  • Terry, K., Williams, S. M., Connolly, L., Ottemann, K. M. (2005). Chemotaxis Plays Multiple Roles during Helicobacter pylori Animal Infection. Infect. Immun. 73: 803-811 [Abstract] [Full Text]  
  • Brahmachary, P., Dashti, M. G., Olson, J. W., Hoover, T. R. (2004). Helicobacter pylori FlgR Is an Enhancer-Independent Activator of {sigma}54-RNA Polymerase Holoenzyme. J. Bacteriol. 186: 4535-4542 [Abstract] [Full Text]  
  • Carrillo, C. D., Taboada, E., Nash, J. H. E., Lanthier, P., Kelly, J., Lau, P. C., Verhulp, R., Mykytczuk, O., Sy, J., Findlay, W. A., Amoako, K., Gomis, S., Willson, P., Austin, J. W., Potter, A., Babiuk, L., Allan, B., Szymanski, C. M. (2004). Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA. J. Biol. Chem. 279: 20327-20338 [Abstract] [Full Text]  
  • Merkx-Jacques, A., Obhi, R. K., Bethune, G., Creuzenet, C. (2004). The Helicobacter pylori flaA1 and wbpB Genes Control Lipopolysaccharide and Flagellum Synthesis and Function. J. Bacteriol. 186: 2253-2265 [Abstract] [Full Text]  
  • Merrell, D. S., Goodrich, M. L., Otto, G., Tompkins, L. S., Falkow, S. (2003). pH-Regulated Gene Expression of the Gastric Pathogen Helicobacter pylori. Infect. Immun. 71: 3529-3539 [Abstract] [Full Text]  
  • Kavermann, H., Burns, B. P., Angermuller, K., Odenbreit, S., Fischer, W., Melchers, K., Haas, R. (2003). Identification and Characterization of Helicobacter pylori Genes Essential for Gastric Colonization. JEM 197: 813-822 [Abstract] [Full Text]  
  • McGEE, D. J., COKER, C., TESTERMAN, T. L., HARRO, J. M., GIBSON, S. V., MOBLEY, H. L. T. (2002). The Helicobacter pylori flbA flagellar biosynthesis and regulatory gene is required for motility and virulence and modulates urease of H. pylori and Proteus mirabilis. J Med Microbiol 51: 958-970 [Abstract] [Full Text]  
  • Andermann, T. M., Chen, Y.-T., Ottemann, K. M. (2002). Two Predicted Chemoreceptors of Helicobacter pylori Promote Stomach Infection. Infect. Immun. 70: 5877-5881 [Abstract] [Full Text]  
  • Guo, B. P., Mekalanos, J. J. (2002). Rapid genetic analysis of Helicobacter pylori gastric mucosal colonization in suckling mice. Proc. Natl. Acad. Sci. USA 99: 8354-8359 [Abstract] [Full Text]  
  • Marchetti, M., Rappuoli, R. (2002). Isogenic mutants of the cag pathogenicity island of Helicobacter pylori in the mouse model of infection: effects on colonization efficiency. Microbiology 148: 1447-1456 [Abstract] [Full Text]  
  • Ottemann, K. M., Lowenthal, A. C. (2002). Helicobacter pylori Uses Motility for Initial Colonization and To Attain Robust Infection. Infect. Immun. 70: 1984-1990 [Abstract] [Full Text]  
  • Tasteyre, A., Barc, M.-C., Collignon, A., Boureau, H., Karjalainen, T. (2001). Role of FliC and FliD Flagellar Proteins of Clostridium difficile in Adherence and Gut Colonization. Infect. Immun. 69: 7937-7940 [Abstract] [Full Text]  
  • Rabaan, A. A., Gryllos, I., Tomas, J. M., Shaw, J. G. (2001). Motility and the Polar Flagellum Are Required for Aeromonas caviae Adherence to HEp-2 Cells. Infect. Immun. 69: 4257-4267 [Abstract] [Full Text]  
  • Tasteyre, A., Karjalainen, T., Avesani, V., Delmée, M., Collignon, A., Bourlioux, P., Barc, M.-C. (2001). Molecular Characterization of fliD Gene Encoding Flagellar Cap and Its Expression among Clostridium difficile Isolates from Different Serogroups. J. Clin. Microbiol. 39: 1178-1183 [Abstract] [Full Text]  
  • Tendeng, C., Badaut, C., Krin, E., Gounon, P., Ngo, S., Danchin, A., Rimsky, S., Bertin, P. (2000). Isolation and Characterization of vicH, Encoding a New Pleiotropic Regulator in Vibrio cholerae. J. Bacteriol. 182: 2026-2032 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»