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Journal of Bacteriology, November 1999, p. 7028-7033, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transcriptional Control of the Low-Temperature-Inducible des Gene, Encoding the Delta 5 Desaturase of Bacillus subtilis

Pablo S. Aguilar,1 Paloma Lopez,2 and Diego de Mendoza1,*

Instituto de Biología Molecular y Celular de Rosario (IBR) and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2000-Rosario, Argentina,1 and Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Científicas, 28006 Madrid, Spain2

Received 14 June 1999/Accepted 13 September 1999

The Bacillus subtilis des gene encodes the cold-inducible Delta 5 lipid desaturase involved in the formation of unsaturated fatty acids from saturated phospholipid precursors. Here, we describe the expression pattern of the des gene in response to a temperature downshift from 37 to 20°C. We found that the synthesis of des mRNA is undetectable at 37°C but dramatically induced upon the temperature downshift. Decay characteristics of the des transcript as well as the in vivo decay of B. subtilis bulk mRNA were investigated. The results showed that the stability of the des transcript as well as of bulk mRNA lasted substantially longer at 20°C than at 37°C. Functional expression of des at 37°C was achieved by exchanging its promoter with the non-cold shock spac promoter. These data provide the first direct evidence that temperature-mediated control of transcription is the major mechanism regulating the mRNA levels of the B. subtilis desaturase. The present results also demonstrate that the only component of the desaturation system regulated by temperature is the desaturase enzyme.

* Corresponding author. Mailing address: Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000-Rosario, Argentina. Phone: 54-341-4350596. Fax: 54-341-4390465. E-mail: diegonet{at}citynet.net.ar.

Journal of Bacteriology, November 1999, p. 7028-7033, Vol. 181, No. 22
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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