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Journal of Bacteriology, December 1999, p. 7629-7633, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Conditional Sigma Factor Expression, Using the Inducible Acetamidase Promoter, Reveals that the Mycobacterium tuberculosis sigF Gene Modulates Expression of the 16-Kilodalton Alpha-Crystallin Homologue

Yukari C. Manabe,1,* Jong Min Chen,2 Chiew G. Ko,3 Ping Chen,3 and William R. Bishai1,2,3

Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine,1 and Department of Microbiology and Molecular Immunology,2 and Center for Tuberculosis Research, Department of International Health,3 Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland

Received 1 July 1999/Accepted 4 October 1999

A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.

* Corresponding author. Mailing address: Center for Tuberculosis Research, Department of Medicine, Division of Infectious Diseases, Johns Hopkins University, 1830 E. Monument St., Room 455, Baltimore, MD 21205.

Journal of Bacteriology, December 1999, p. 7629-7633, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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