This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lloyd, J. R.
Right arrow Articles by Lovley, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lloyd, J. R.
Right arrow Articles by Lovley, D. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 1999, p. 7647-7649, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Periplasmic 9.6-Kilodalton c-Type Cytochrome of Geobacter sulfurreducens Is Not an Electron Shuttle to Fe(III)

Jon R. Lloyd, Elizabeth L. Blunt-Harris, and Derek R. Lovley*

Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003

Received 12 August 1999/Accepted 30 September 1999

Geobacter sulfurreducens contains a 9.6-kDa c-type cytochrome that was previously proposed to serve as an extracellular electron shuttle to insoluble Fe(III) oxides. However, when the cytochrome was added to washed-cell suspensions of G. sulfurreducens it did not enhance Fe(III) oxide reduction, whereas similar concentrations of the known electron shuttle, anthraquinone-2,6-disulfonate, greatly stimulated Fe(III) oxide reduction. Furthermore, analysis of the extracellular c-type cytochromes in cultures of G. sulfurreducens demonstrated that the dominant c-type cytochrome was not the 9.6-kDa cytochrome, but rather a 41-kDa cytochrome. These results and other considerations suggest that the 9.6-kDa cytochrome is not an important extracellular electron shuttle to Fe(III) oxides.

* Corresponding author. Mailing address: Department of Microbiology, University of Massachusetts, Amherst, MA 01003. Phone: (413) 545-9651. Fax: (413) 545-1578. E-mail: dlovley{at}microbio.umass.edu.

Journal of Bacteriology, December 1999, p. 7647-7649, Vol. 181, No. 24
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mahadevan, R., Bond, D. R., Butler, J. E., Esteve-Nunez, A., Coppi, M. V., Palsson, B. O., Schilling, C. H., Lovley, D. R. (2006). Characterization of Metabolism in the Fe(III)-Reducing Organism Geobacter sulfurreducens by Constraint-Based Modeling. Appl. Environ. Microbiol. 72: 1558-1568 [Abstract] [Full Text]  
  • Mehta, T., Coppi, M. V., Childers, S. E., Lovley, D. R. (2005). Outer Membrane c-Type Cytochromes Required for Fe(III) and Mn(IV) Oxide Reduction in Geobacter sulfurreducens. Appl. Environ. Microbiol. 71: 8634-8641 [Abstract] [Full Text]  
  • Fayek, M., Utsunomiya, S., Pfiffner, S. M., White, D. C., Riciputi, L. R., Ewing, R. C., Anovitz, L. M., Stadermann, F. J. (2005). THE APPLICATION OF HRTEM TECHNIQUES AND NANOSIMS TO CHEMICALLY AND ISOTOPICALLY CHARACTERIZE GEOBACTER SULFURREDUCENS SURFACES. Can Mineral 43: 1631-1641 [Abstract] [Full Text]  
  • Johnson, C. M., Beard, B. L., Roden, E. E., Newman, D. K., Nealson, K. H. (2004). Isotopic Constraints on Biogeochemical Cycling of Fe. Reviews in Mineralogy and Geochemistry 55: 359-408 [Full Text]  
  • Nevin, K. P., Lovley, D. R. (2002). Mechanisms for Accessing Insoluble Fe(III) Oxide during Dissimilatory Fe(III) Reduction by Geothrix fermentans. Appl. Environ. Microbiol. 68: 2294-2299 [Abstract] [Full Text]  
  • Turick, C. E., Tisa, L. S., Caccavo, F. Jr. (2002). Melanin Production and Use as a Soluble Electron Shuttle for Fe(III) Oxide Reduction and as a Terminal Electron Acceptor by Shewanella algae BrY. Appl. Environ. Microbiol. 68: 2436-2444 [Abstract] [Full Text]  
  • DiChristina, T. J., Moore, C. M., Haller, C. A. (2002). Dissimilatory Fe(III) and Mn(IV) Reduction by Shewanella putrefaciens Requires ferE, a Homolog of the pulE (gspE) Type II Protein Secretion Gene. J. Bacteriol. 184: 142-151 [Abstract] [Full Text]  
  • Kaufmann, F., Lovley, D. R. (2001). Isolation and Characterization of a Soluble NADPH-Dependent Fe(III) Reductase from Geobacter sulfurreducens. J. Bacteriol. 183: 4468-4476 [Abstract] [Full Text]  
  • Lloyd, J. R., Sole, V. A., Van Praagh, C. V. G., Lovley, D. R. (2000). Direct and Fe(II)-Mediated Reduction of Technetium by Fe(III)-Reducing Bacteria. Appl. Environ. Microbiol. 66: 3743-3749 [Abstract] [Full Text]  
  • Nevin, K. P., Lovley, D. R. (2000). Lack of Production of Electron-Shuttling Compounds or Solubilization of Fe(III) during Reduction of Insoluble Fe(III) Oxide by Geobacter metallireducens. Appl. Environ. Microbiol. 66: 2248-2251 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»