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Journal of Bacteriology, February 1999, p. 1324-1329, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Enterococcus faecalis pyr Operon Is Regulated by Autogenous Transcriptional Attenuation at a Single Site in the 5' Leader

Sa-Youl Ghim,dagger Charles C. Kim,Dagger Eric R. Bonner, John N. D'Elia, Gail K. Grabner, and Robert L. Switzer*

Department of Biochemistry, University of Illinois, Urbana, Illinois 61801

Received 3 August 1998/Accepted 11 December 1998

The 5' end of the Enterococcus faecalis pyr operon specifies, in order, the promoter, a 5' untranslated leader, the pyrR gene encoding the regulatory protein for the operon, a 39-nucleotide (nt) intercistronic region, the pyrP gene encoding a uracil permease, a 13-nt intercistronic region, and the pyrB gene encoding aspartate transcarbamylase. The 5' leader RNA is capable of forming stem-loop structures involved in attenuation control of the operon. No attenuation regions, such as those found in the Bacillus subtilis pyr operon, are present in the pyrR-pyrP or pyrP-pyrB intercistronic regions. Several lines of evidence demonstrate that the E. faecalis pyr operon is repressed by uracil via transcriptional attenuation at the single 5' leader termination site and that attenuation is mediated by the PyrR protein.

* Corresponding author. Mailing address: Department of Biochemistry, University of Illinois, 600 South Mathews Ave., Urbana, IL 61801. Phone: (217) 333-3940. Fax: (217) 244-5858. E-mail: rswitzer{at}uiuc.edu.

dagger Present address: Department of Biology, Teacher's College, KyungPook National University, Buk-Gu, Taegu 702-701, Republic of Korea.

Dagger Present address: Department of Microbiology and Immunology, Stanford University Medical Center, Stanford, CA 94305.

Journal of Bacteriology, February 1999, p. 1324-1329, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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