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Journal of Bacteriology, February 1999, p. 1330-1333, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

sn-Glycerol-1-Phosphate-Forming Activities in Archaea: Separation of Archaeal Phospholipid Biosynthesis and Glycerol Catabolism by Glycerophosphate Enantiomers

Masateru Nishihara,1 Tomoko Yamazaki,2 Tairo Oshima,2 and Yosuke Koga1,*

Department of Chemistry, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555,1 and Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392,2 Japan

Received 24 August 1998/Accepted 7 December 1998

In Methanobacterium thermoautotrophicum, sn-glycerol-1-phosphate (G-1-P) dehydrogenase is responsible for the formation of the Archaea-specific backbone of phospholipids, G-1-P, from dihydroxyacetonephosphate (DHAP). The possible G-1-P-forming activities were surveyed in cell-free extracts of six species of Archaea. All the archaeal cell-free homogenates tested revealed the ability to form G-1-P from DHAP. In addition, activities of G-3-P-forming glycerol kinase and G-3-P dehydrogenase were also detected in four heterotrophic archaea, while glycerol kinase activity was not detected in two autotrophic methanogens. These results show that G-1-P is produced from DHAP by G-1-P dehydrogenase in a wide variety of archaea while exogenous glycerol is catabolized via G-3-P.

* Corresponding author. Mailing address: Department of Chemistry, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555 Japan. Phone: 81-93-691-7215. Fax: 81-93-693-9921. E-mail: kogay{at}med.uoeh-u.ac.jp.

Journal of Bacteriology, February 1999, p. 1330-1333, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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