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Journal of Bacteriology, February 1999, p. 1360-1363, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Chromosomal Changes during Experimental Evolution in Laboratory Populations of Escherichia coli

Ulfar Bergthorsson1,2 and Howard Ochman1,3,*

Department of Biology, University of Rochester, Rochester, New York 14627,1 Department of Biology, University of Utah, Salt Lake City, Utah 84112,2 and Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 857213

Received 1 June 1998/Accepted 7 December 1998

Short-term rates of chromosome evolution were analyzed in experimental populations of Escherichia coli B that had been propagated for 2,000 generations under four thermal regimens. Chromosome alterations were monitored in 24 independent populations by pulsed-field gel electrophoresis of DNA treated with five rare-cutting restriction enzymes. A total of 11 changes, 8 affecting chromosome size and 3 altering restriction sites, were observed in these populations, with none occurring in strains cultured at 37°C. Considering the changes detected in these experimental populations, the rate of chromosome alteration of E. coli is estimated to be half of that observed in experimental populations of yeast.

* Corresponding author. Mailing address: Department of Ecology and Evolutionary Biology, 233 Life Sciences South, University of Arizona, Tucson, AZ 85721. Phone: (520) 626-8355. Fax: (520) 621-3709. E-mail: hochman{at}u.arizona.edu.

Journal of Bacteriology, February 1999, p. 1360-1363, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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