This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stöver, A. G.
Right arrow Articles by Driks, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stöver, A. G.
Right arrow Articles by Driks, A.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 1999, p. 1664-1672, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Secretion, Localization, and Antibacterial Activity of TasA, a Bacillus subtilis Spore-Associated Protein

Axel G. Stöver and Adam Driks*

Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, Illinois 60153

Received 2 October 1998/Accepted 22 December 1998

The synthesis and subcellular localization of the proteins that comprise the Bacillus subtilis spore are under a variety of complex controls. To better understand these controls, we have identified and characterized a 31-kDa sporulation protein, called TasA, which is secreted into the culture medium early in sporulation and is also incorporated into the spore. TasA synthesis begins approximately 30 min after the onset of sporulation and requires the sporulation transcription factor genes spo0H and spo0A. The first 81 nucleotides of tasA encode a 27-amino-acid sequence that resembles a signal peptide and which is missing from TasA isolated from a sporulating cell lysate. In B. subtilis cells unable to synthesize the signal peptidase SipW, TasA is not secreted, nor is it incorporated into spores. Cells unable to produce SipW produce a 34-kDa form of TasA, consistent with a failure to remove the N-terminal 27 amino acids. In cells engineered to express sipW and tasA during exponential growth, TasA migrates as a 31-kDa species and is secreted into the culture medium. These results indicate that SipW plays a crucial role in the export of TasA out of the cell and its incorporation into spores. Although TasA is dispensable for sporulation under laboratory conditions, we find that TasA has a broad-spectrum antibacterial activity. We discuss the possibility that during the beginning of sporulation as well as later, during germination, TasA inhibits other organisms in the environment, thus conferring a competitive advantage to the spore.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Loyola University Medical Center, 2160 South First Ave., Maywood, IL 60153. Phone: (708) 216-3706. Fax: (708) 216-9574. E-mail: adriks{at}luc.edu.

Journal of Bacteriology, March 1999, p. 1664-1672, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Winkelman, J. T., Blair, K. M., Kearns, D. B. (2009). RemA (YlzA) and RemB (YaaB) Regulate Extracellular Matrix Operon Expression and Biofilm Formation in Bacillus subtilis. J. Bacteriol. 191: 3981-3991 [Abstract] [Full Text]  
  • Serrano, M., Vieira, F., Moran, C. P. Jr., Henriques, A. O. (2008). Processing of a Membrane Protein Required for Cell-to-Cell Signaling during Endospore Formation in Bacillus subtilis. J. Bacteriol. 190: 7786-7796 [Abstract] [Full Text]  
  • Vlamakis, H., Aguilar, C., Losick, R., Kolter, R. (2008). Control of cell fate by the formation of an architecturally complex bacterial community. Genes Dev. 22: 945-953 [Abstract] [Full Text]  
  • Stein, C., Jones, G. W., Chalmers, T., Berry, C. (2006). Transcriptional Analysis of the Toxin-Coding Plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis.. Appl. Environ. Microbiol. 72: 1771-1776 [Abstract] [Full Text]  
  • Leclere, V., Bechet, M., Adam, A., Guez, J.-S., Wathelet, B., Ongena, M., Thonart, P., Gancel, F., Chollet-Imbert, M., Jacques, P. (2005). Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities. Appl. Environ. Microbiol. 71: 4577-4584 [Abstract] [Full Text]  
  • Branda, S. S., Gonzalez-Pastor, J. E., Dervyn, E., Ehrlich, S. D., Losick, R., Kolter, R. (2004). Genes Involved in Formation of Structured Multicellular Communities by Bacillus subtilis. J. Bacteriol. 186: 3970-3979 [Abstract] [Full Text]  
  • Tjalsma, H., Antelmann, H., Jongbloed, J. D.H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., Kuipers, O. P., Bron, S., Hecker, M., van Dijl, J. M. (2004). Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome. Microbiol. Mol. Biol. Rev. 68: 207-233 [Abstract] [Full Text]  
  • Rhee, K.-J., Sethupathi, P., Driks, A., Lanning, D. K., Knight, K. L. (2004). Role of Commensal Bacteria in Development of Gut-Associated Lymphoid Tissues and Preimmune Antibody Repertoire. J. Immunol. 172: 1118-1124 [Abstract] [Full Text]  
  • Williams, R. C., Rees, M. L., Jacobs, M. F., Pragai, Z., Thwaite, J. E., Baillie, L. W. J., Emmerson, P. T., Harwood, C. R. (2003). Production of Bacillus anthracis Protective Antigen Is Dependent on the Extracellular Chaperone, PrsA. J. Biol. Chem. 278: 18056-18062 [Abstract] [Full Text]  
  • Inaoka, T., Takahashi, K., Ohnishi-Kameyama, M., Yoshida, M., Ochi, K. (2003). Guanine Nucleotides Guanosine 5'-Diphosphate 3'-Diphosphate and GTP Co-operatively Regulate the Production of an Antibiotic Bacilysin in Bacillus subtilis. J. Biol. Chem. 278: 2169-2176 [Abstract] [Full Text]  
  • Berry, C., O'Neil, S., Ben-Dov, E., Jones, A. F., Murphy, L., Quail, M. A., Holden, M. T. G., Harris, D., Zaritsky, A., Parkhill, J. (2002). Complete Sequence and Organization of pBtoxis, the Toxin-Coding Plasmid of Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 68: 5082-5095 [Abstract] [Full Text]  
  • Tamehiro, N., Okamoto-Hosoya, Y., Okamoto, S., Ubukata, M., Hamada, M., Naganawa, H., Ochi, K. (2002). Bacilysocin, a Novel Phospholipid Antibiotic Produced by Bacillus subtilis 168. Antimicrob. Agents Chemother. 46: 315-320 [Abstract] [Full Text]  
  • van Roosmalen, M. L., Jongbloed, J. D. H., Jong, A. d., van Eerden, J., Venema, G., Bron, S., Maarten van Dijl, J. (2001). Detergent-independent in vitro activity of a truncated Bacillus signal peptidase. Microbiology 147: 909-917 [Abstract] [Full Text]  
  • Catalano, F. A., Meador-Parton, J., Popham, D. L., Driks, A. (2001). Amino Acids in the Bacillus subtilis Morphogenetic Protein SpoIVA with Roles in Spore Coat and Cortex Formation. J. Bacteriol. 183: 1645-1654 [Abstract] [Full Text]  
  • Tjalsma, H., Bolhuis, A., Jongbloed, J. D. H., Bron, S., van Dijl, J. M. (2000). Signal Peptide-Dependent Protein Transport in Bacillus subtilis: a Genome-Based Survey of the Secretome. Microbiol. Mol. Biol. Rev. 64: 515-547 [Abstract] [Full Text]  
  • Ozin, A. J., Henriques, A. O., Yi, H., Moran, C. P. Jr. (2000). Morphogenetic Proteins SpoVID and SafA Form a Complex during Assembly of the Bacillus subtilis Spore Coat. J. Bacteriol. 182: 1828-1833 [Abstract] [Full Text]  
  • Hirose, I., Sano, K., Shioda, I., Kumano, M., Nakamura, K., Yamane, K. (2000). Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study. Microbiology 146: 65-75 [Abstract] [Full Text]  
  • Bauer, T., Little, S., Stöver, A. G., Driks, A. (1999). Functional Regions of the Bacillus subtilis Spore Coat Morphogenetic Protein CotE. J. Bacteriol. 181: 7043-7051 [Abstract] [Full Text]  
  • Stöver, A. G., Driks, A. (1999). Control of Synthesis and Secretion of the Bacillus subtilis Protein YqxM. J. Bacteriol. 181: 7065-7069 [Abstract] [Full Text]  
  • Stöver, A. G., Driks, A. (1999). Regulation of Synthesis of the Bacillus subtilis Transition-Phase, Spore-Associated Antibacterial Protein TasA. J. Bacteriol. 181: 5476-5481 [Abstract] [Full Text]  
  • Serrano, M., Zilhão, R., Ricca, E., Ozin, A. J., Moran, C. P. Jr., Henriques, A. O. (1999). A Bacillus subtilis Secreted Protein with a Role in Endospore Coat Assembly and Function. J. Bacteriol. 181: 3632-3643 [Abstract] [Full Text]  
  • Tjalsma, H., Stover, A. G., Driks, A., Venema, G., Bron, S., van Dijl, J. M. (2000). Conserved Serine and Histidine Residues Are Critical for Activity of the ER-type Signal Peptidase SipW of Bacillus subtilis. J. Biol. Chem. 275: 25102-25108 [Abstract] [Full Text]  
  • van Roosmalen, M. L., Jongbloed, J. D. H., Dubois, J.-Y. F., Venema, G., Bron, S., van Dijl, J. M. (2001). Distinction between Major and Minor Bacillus Signal Peptidases Based on Phylogenetic and Structural Criteria. J. Biol. Chem. 276: 25230-25235 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»