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Journal of Bacteriology, March 1999, p. 1689-1693, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Functional Importance and Local Environments of the Cysteines in the Tetracycline Resistance Protein Encoded by Plasmid pBR322

Jean E. Jewell, Jill Orwick, Jun Liu, and Kurt W. Miller*

Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071

Received 22 September 1998/Accepted 17 December 1998

The properties of the cysteines in the pBR322-encoded tetracycline resistance protein have been examined. Cysteines are important but not essential for tetracycline transport activity. None of the cysteines reacted with biotin maleimide, suggesting that they are shielded from the aqueous phase or reside in a negatively charged local environment.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Wyoming, P.O. Box 3944, Laramie, WY 82071-3944. Phone: (307) 766-2037. Fax: (307) 766-5098. E-mail: kwmiller{at}uwyo.edu.

Journal of Bacteriology, March 1999, p. 1689-1693, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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