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Journal of Bacteriology, March 1999, p. 1868-1874, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Hypothesis Testing with Candida albicans through Gene Disruption with Short Homology Regions

R. Bryce Wilson, Dana Davis, and Aaron P. Mitchell*

Department of Microbiology and Institute of Cancer Research, Columbia University, New York, New York 10032

Received 5 November 1998/Accepted 6 January 1999

Disruption of newly identified genes in the pathogen Candida albicans is a vital step in determination of gene function. Several gene disruption methods described previously employ long regions of homology flanking a selectable marker. Here, we describe disruption of C. albicans genes with PCR products that have 50 to 60 bp of homology to a genomic sequence on each end of a selectable marker. We used the method to disrupt two known genes, ARG5 and ADE2, and two sequences newly identified through the Candida genome project, HRM101 and ENX3. HRM101 and ENX3 are homologous to genes in the conserved RIM101 (previously called RIM1) and PacC pathways of Saccharomyces cerevisiae and Aspergillus nidulans. We show that three independent hrm101/hrm101 mutants and two independent enx3/enx3 mutants are defective in filamentation on Spider medium. These observations argue that HRM101 and ENX3 sequences are indeed portions of genes and that the respective gene products have related functions.

* Corresponding author. Mailing address: Department of Microbiology and Institute of Cancer Research, Columbia University, 701 West 168th St., New York, NY 10032. Phone: (212) 305-8251. Fax: (212) 305-1741. E-mail: apm4{at}columbia.edu.

Journal of Bacteriology, March 1999, p. 1868-1874, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Toenjes, K. A., Munsee, S. M., Ibrahim, A. S., Jeffrey, R., Edwards, J. E. Jr., Johnson, D. I. (2005). Small-Molecule Inhibitors of the Budded-to-Hyphal-Form Transition in the Pathogenic Yeast Candida albicans. Antimicrob. Agents Chemother. 49: 963-972 [Abstract] [Full Text]  
  • Bassilana, M., Hopkins, J., Arkowitz, R. A. (2005). Regulation of the Cdc42/Cdc24 GTPase Module during Candida albicans Hyphal Growth. Eukaryot Cell 4: 588-603 [Abstract] [Full Text]  
  • Noble, S. M., Johnson, A. D. (2005). Strains and Strategies for Large-Scale Gene Deletion Studies of the Diploid Human Fungal Pathogen Candida albicans. Eukaryot Cell 4: 298-309 [Abstract] [Full Text]  
  • Chapa y Lazo, B., Bates, S., Sudbery, P. (2005). The G1 Cyclin Cln3 Regulates Morphogenesis in Candida albicans. Eukaryot Cell 4: 90-94 [Abstract] [Full Text]  
  • Chen, C.-G., Yang, Y.-L., Shih, H.-I, Su, C.-L., Lo, H.-J. (2004). CaNdt80 Is Involved in Drug Resistance in Candida albicans by Regulating CDR1. Antimicrob. Agents Chemother. 48: 4505-4512 [Abstract] [Full Text]  
  • Onyewu, C., Wormley, F. L. Jr., Perfect, J. R., Heitman, J. (2004). The Calcineurin Target, Crz1, Functions in Azole Tolerance but Is Not Required for Virulence of Candida albicans. Infect. Immun. 72: 7330-7333 [Abstract] [Full Text]  
  • Silver, P. M., Oliver, B. G., White, T. C. (2004). Role of Candida albicans Transcription Factor Upc2p in Drug Resistance and Sterol Metabolism. Eukaryot Cell 3: 1391-1397 [Abstract] [Full Text]  
  • Martin, R., Walther, A., Wendland, J. (2004). Deletion of the Dynein Heavy-Chain Gene DYN1 Leads to Aberrant Nuclear Positioning and Defective Hyphal Development in Candida albicans. Eukaryot Cell 3: 1574-1588 [Abstract] [Full Text]  
  • Kullas, A. L., Li, M., Davis, D. A. (2004). Snf7p, a Component of the ESCRT-III Protein Complex, Is an Upstream Member of the RIM101 Pathway in Candida albicans. Eukaryot Cell 3: 1609-1618 [Abstract] [Full Text]  
  • Xu, W., Smith, F. J. Jr., Subaran, R., Mitchell, A. P. (2004). Multivesicular Body-ESCRT Components Function in pH Response Regulation in Saccharomyces cerevisiae and Candida albicans. Mol. Biol. Cell 15: 5528-5537 [Abstract] [Full Text]  
  • Martinez, A. I., Castillo, L., Garcera, A., Elorza, M. V., Valentin, E., Sentandreu, R. (2004). Role of Pir1 in the construction of the Candida albicans cell wall. Microbiology 150: 3151-3161 [Abstract] [Full Text]  
  • Martin, S. W., Konopka, J. B. (2004). SUMO Modification of Septin-interacting Proteins in Candida albicans. J. Biol. Chem. 279: 40861-40867 [Abstract] [Full Text]  


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