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Journal of Bacteriology, April 1999, p. 2257-2260, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Induction of Prophages of Enterohemorrhagic Escherichia coli O157:H7 with Norfloxacin

Aizo Matsushiro,1,* Koki Sato,1 Hiroshi Miyamoto,1 Tadashi Yamamura,1 and Takeshi Honda2

The School of Biology-Oriented Science and Technology, Department of Molecular Genetics, Kinki University, Wakayama 649-6493,1 and The Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871,2 Japan

Received 8 July 1998/Accepted 25 January 1999

Norfloxacin (NFLX) caused induction of prophages VT1 and VT2 of enterohemorrhagic Escherichia coli O157 at subinhibitory concentrations. In time course experiments, we observed the following sequential events: upon induction, the phage genomes underwent multiplication; the amount of stx genes increased; and subsequently, large quantities of toxins VT1 and VT2 were produced. Further studies showed that the molecular mechanism of prophage induction is closely related to the RecA system since the prophage VT2 was not induced with NFLX in a recA mutant strain.

* Corresponding author. Mailing address: The School of Biology-Oriented Science and Technology, Department of Molecular Genetics, Kinki University, Wakayama 649-6493, Japan. Phone: 81-736-77-0345, ext. 4408. Fax: 81-736-77-4754. E-mail: miyamoto{at}bio.waka.kindai.ac.jp.

Journal of Bacteriology, April 1999, p. 2257-2260, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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