This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deng, W.-L.
Right arrow Articles by Huang, H.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deng, W.-L.
Right arrow Articles by Huang, H.-C.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 1999, p. 2298-2301, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cellular Locations of Pseudomonas syringae pv. syringae HrcC and HrcJ Proteins, Required for Harpin Secretion via the Type III Pathway

Wen-Ling Dengdagger and Hsiou-Chen Huang*

Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan

Received 6 October 1998/Accepted 22 January 1999

The complete hrp-hrc-hrmA cluster of Pseudomonas syringae pv. syringae 61 encodes 28 polypeptides. A saprophytic bacterium carrying this cluster is capable of secreting HrpZ---a harpin encoded by hrpZ---in an hrp-dependent manner, which suggests that this cluster contains sufficient components to assemble functional type III secretion machinery. Sequence data show that HrcJ and HrcC are putative outer membrane proteins, and nonpolar mutagenesis demonstrates they are all required for HrpZ secretion. In this study, we investigated the cellular localization of the HrcC and HrcJ proteins by Triton solubilization, sucrose-gradient isopycnic centrifugation, and immunogold labeling of the bacterial cell surface. Our results indicate that HrcC is indeed an outer membrane protein and that HrcJ is located between both membranes. Their membrane localization suggests that they might be involved in the formation of a supramolecular structure for protein secretion.

* Corresponding author. Mailing address: Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan. Phone: 886-4-2852155. Fax: 886-4-2861905. E-mail: hchuang{at}dragon.nchu.edu.tw.

dagger Present address: Department of Plant Pathology, Cornell University, Ithaca, NY 14853-4203.

Journal of Bacteriology, April 1999, p. 2298-2301, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Xing, D. H., Lai, Z. B., Zheng, Z. Y., Vinod, K. M., Fan, B. F., Chen, Z. X. (2008). Stress- and Pathogen-Induced Arabidopsis WRKY48 is a Transcriptional Activator that Represses Plant Basal Defense. Mol Plant 0: ssn020v1-ssn020 [Abstract] [Full Text]  
  • Kostakioti, M., Newman, C. L., Thanassi, D. G., Stathopoulos, C. (2005). Mechanisms of Protein Export across the Bacterial Outer Membrane. J. Bacteriol. 187: 4306-4314 [Full Text]  
  • Garmendia, J., Frankel, G., Crepin, V. F. (2005). Enteropathogenic and Enterohemorrhagic Escherichia coli Infections: Translocation, Translocation, Translocation. Infect. Immun. 73: 2573-2585 [Full Text]  
  • Gauthier, A., Thomas, N. A., Finlay, B. B. (2003). Bacterial Injection Machines. J. Biol. Chem. 278: 25273-25276 [Full Text]  
  • Gauthier, A., Puente, J. L., Finlay, B. B. (2003). Secretin of the Enteropathogenic Escherichia coli Type III Secretion System Requires Components of the Type III Apparatus for Assembly and Localization. Infect. Immun. 71: 3310-3319 [Abstract] [Full Text]  
  • Collmer, A., Badel, J. L., Charkowski, A. O., Deng, W.-L., Fouts, D. E., Ramos, A. R., Rehm, A. H., Anderson, D. M., Schneewind, O., van Dijk, K., Alfano, J. R. (2000). Pseudomonas syringae Hrp type III secretion system and effector proteins. Proc. Natl. Acad. Sci. USA 97: 8770-8777 [Abstract] [Full Text]  
  • Cornelis, G. R. (2000). Molecular and cell biology aspects of plague. Proc. Natl. Acad. Sci. USA 97: 8778-8783 [Abstract] [Full Text]  
  • Wei, W., Plovanich-Jones, A., Deng, W.-L., Jin, Q.-L., Collmer, A., Huang, H.-C., He, S. Y. (2000). The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato. Proc. Natl. Acad. Sci. USA 97: 2247-2252 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»