This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chauvaux, S.
Right arrow Articles by Beguin, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chauvaux, S.
Right arrow Articles by Beguin, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 1999, p. 2455-2458, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Distinct Affinity of Binding Sites for S-Layer Homologous Domains in Clostridium thermocellum and Bacillus anthracis Cell Envelopes

Sylvie Chauvaux,* Markus Matuschek,dagger and Pierre Beguin

Unité de Physiologie Cellulaire, Département des Biotechnologies, Institut Pasteur, 75724 Paris Cedex 15, France

Received 28 September 1998/Accepted 1 February 1999

Binding parameters were determined for the SLH (S-layer homologous) domains from the Clostridium thermocellum outer layer protein OlpB, from the C. thermocellum S-layer protein SlpA, and from the Bacillus anthracis S-layer proteins EA1 and Sap, using cell walls from C. thermocellum and B. anthracis. Each SLH domain bound to C. thermocellum and B. anthracis cell walls with a different KD, ranging between 7.1 × 10-7 and 1.8 × 10-8 M. Cell wall binding sites for SLH domains displayed different binding specificities in C. thermocellum and B. anthracis. SLH-binding sites were not detected in cell walls of Bacillus subtilis. Cell walls of C. thermocellum lost their affinity for SLH domains after treatment with 48% hydrofluoric acid but not after treatment with formamide or dilute acid. A soluble component, extracted from C. thermocellum cells by sodium dodecyl sulfate treatment, bound the SLH domains from C. thermocellum but not those from B. anthracis proteins. A corresponding component was not found in B. anthracis.

* Corresponding author. Mailing address: Unité de Physiologie Cellulaire, Département des Biotechnologies, Institut Pasteur, 25, rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33 1 40 61 37 04. Fax: 33 1 45 68 87 90. E-mail: chauvaux{at}pasteur.fr.

dagger Present address: ZHF/D-A30, BASF Aktiengesellschaft, 67056 Ludwigshafen, Germany.

Journal of Bacteriology, April 1999, p. 2455-2458, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ferner-Ortner, J., Mader, C., Ilk, N., Sleytr, U. B., Egelseer, E. M. (2007). High-Affinity Interaction between the S-Layer Protein SbsC and the Secondary Cell Wall Polymer of Geobacillus stearothermophilus ATCC 12980 Determined by Surface Plasmon Resonance Technology. J. Bacteriol. 189: 7154-7158 [Abstract] [Full Text]  
  • Demain, A. L., Newcomb, M., Wu, J. H. D. (2005). Cellulase, Clostridia, and Ethanol. Microbiol. Mol. Biol. Rev. 69: 124-154 [Abstract] [Full Text]  
  • Xu, Q., Barak, Y., Kenig, R., Shoham, Y., Bayer, E. A., Lamed, R. (2004). A Novel Acetivibrio cellulolyticus Anchoring Scaffoldin That Bears Divergent Cohesins. J. Bacteriol. 186: 5782-5789 [Abstract] [Full Text]  
  • Mader, C., Huber, C., Moll, D., Sleytr, U. B., Sara, M. (2004). Interaction of the Crystalline Bacterial Cell Surface Layer Protein SbsB and the Secondary Cell Wall Polymer of Geobacillus stearothermophilus PV72 Assessed by Real-Time Surface Plasmon Resonance Biosensor Technology. J. Bacteriol. 186: 1758-1768 [Abstract] [Full Text]  
  • Vollenkle, C., Weigert, S., Ilk, N., Egelseer, E., Weber, V., Loth, F., Falkenhagen, D., Sleytr, U. B., Sara, M. (2004). Construction of a Functional S-Layer Fusion Protein Comprising an Immunoglobulin G-Binding Domain for Development of Specific Adsorbents for Extracorporeal Blood Purification. Appl. Environ. Microbiol. 70: 1514-1521 [Abstract] [Full Text]  
  • Xu, Q., Gao, W., Ding, S.-Y., Kenig, R., Shoham, Y., Bayer, E. A., Lamed, R. (2003). The Cellulosome System of Acetivibrio cellulolyticus Includes a Novel Type of Adaptor Protein and a Cell Surface Anchoring Protein. J. Bacteriol. 185: 4548-4557 [Abstract] [Full Text]  
  • Calabi, E., Fairweather, N. (2002). Patterns of Sequence Conservation in the S-Layer Proteins and Related Sequences in Clostridium difficile. J. Bacteriol. 184: 3886-3897 [Abstract] [Full Text]  
  • Ilk, N., Vollenkle, C., Egelseer, E. M., Breitwieser, A., Sleytr, U. B., Sara, M. (2002). Molecular Characterization of the S-Layer Gene, sbpA, of Bacillus sphaericus CCM 2177 and Production of a Functional S-Layer Fusion Protein with the Ability To Recrystallize in a Defined Orientation while Presenting the Fused Allergen. Appl. Environ. Microbiol. 68: 3251-3260 [Abstract] [Full Text]  
  • Kosugi, A., Murashima, K., Tamaru, Y., Doi, R. H. (2002). Cell-Surface-Anchoring Role of N-Terminal Surface Layer Homology Domains of Clostridium cellulovorans EngE. J. Bacteriol. 184: 884-888 [Abstract] [Full Text]  
  • Karjalainen, T., Waligora-Dupriet, A.-J., Cerquetti, M., Spigaglia, P., Maggioni, A., Mauri, P., Mastrantonio, P. (2001). Molecular and Genomic Analysis of Genes Encoding Surface-Anchored Proteins from Clostridium difficile. Infect. Immun. 69: 3442-3446 [Abstract] [Full Text]  
  • Jarosch, M., Egelseer, E. M., Huber, C., Moll, D., Mattanovich, D., Sleytr, U. B., Sára, M. (2001). Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms. Microbiology 147: 1353-1363 [Abstract] [Full Text]  
  • Sára, M., Sleytr, U. B. (2000). S-Layer Proteins. J. Bacteriol. 182: 859-868 [Full Text]  
  • Jarosch, M., Egelseer, E. M., Mattanovich, D., Sleytr, U. B., Sára, M. (2000). S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli. Microbiology 146: 273-281 [Abstract] [Full Text]  
  • Ilk, N., Kosma, P., Puchberger, M., Egelseer, E. M., Mayer, H. F., Sleytr, U. B., Sára, M. (1999). Structural and Functional Analyses of the Secondary Cell Wall Polymer of Bacillus sphaericus CCM 2177 That Serves as an S-Layer-Specific Anchor. J. Bacteriol. 181: 7643-7646 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»