This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beeser, A. E.
Right arrow Articles by Cooper, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beeser, A. E.
Right arrow Articles by Cooper, T. G.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 1999, p. 2472-2476, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Control of Nitrogen Catabolite Repression Is Not Affected by the tRNAGln-CUU Mutation, Which Results in Constitutive Pseudohyphal Growth of Saccharomyces cerevisiae

Alexander E. Beeser and Terrance G. Cooper*

Department of Microbiology and Immunology, University of Tennessee, Memphis, Tennessee 38163

Received 8 December 1998/Accepted 9 February 1999

Saccharomyces cerevisiae responds to nitrogen availability in several ways. (i) The cell is able to distinguish good nitrogen sources from poor ones through a process designated nitrogen catabolite repression (NCR). Good and poor nitrogen sources do not demonstrably affect the cell cycle other than to influence the cell's doubling time. (ii) Nitrogen starvation promotes the initiation of sporulation and pseudohyphal growth. (iii) Nitrogen starvation strongly affects the cell cycle; nitrogen-starved cells arrest in G1. A specific allele of the SUP70/CDC65 tRNAGln gene (sup70-65) has been reported to be defective in nitrogen signaling associated with pseudohyphal formation, sporulation, and NCR. Our data confirm that pseudohyphal growth occurs gratuitously in sup70-65 mutants cultured in nitrogen-rich medium at 30°C. However, we find neither any defect in NCR in the sup70-65 mutant nor any alteration in the control of YVH1 expression, which has been previously shown to be specifically induced by nitrogen starvation.

* Corresponding author. Phone: (901) 448-6175. Fax: (901) 448-8462. E-mail: tcooper{at}utmem1.utmem.edu.

Journal of Bacteriology, April 1999, p. 2472-2476, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Parsons, R., Sunley, R. J. (2001). Nitrogen nutrition and the role of root-shoot nitrogen signalling particularly in symbiotic systems. J Exp Bot 52: 435-443 [Abstract] [Full Text]  
  • Cox, K. H., Rai, R., Distler, M., Daugherty, J. R., Coffman, J. A., Cooper, T. G. (2000). Saccharomyces cerevisiae GATA Sequences Function as TATA Elements during Nitrogen Catabolite Repression and When Gln3p Is Excluded from the Nucleus by Overproduction of Ure2p. J. Biol. Chem. 275: 17611-17618 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»