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Journal of Bacteriology, April 1999, p. 2631-2633, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Assembly Requirements and Role of CotH during Spore Coat Formation in Bacillus subtilis

R. Zilhão,1 G. Naclerio,2 A. O. Henriques,3,dagger L. Baccigalupi,4 C. P. Moran Jr.,3 and E. Ricca4,*

Department of Plant Physiology, University of Lisbon, Campo Grande Bl C2, 1700 Lisbon, Portugal1; University of Molise, 86170 Isernia,2 and Department of General and Environmental Physiology, University Federico II, 80134 Naples,4 Italy; and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 303223

Received 30 October 1998/Accepted 10 February 1999

We report Western blot data showing that the 42.8-kDa product of the previously characterized cotH locus (8) is a structural component of the Bacillus subtilis spore coat. We show that the assembly of CotH requires both CotE and GerE. In agreement with these observations, the ultrastructural analysis of purified spores suggests that CotH is needed for proper formation of both inner and outer layers of the coat.

* Corresponding author. Mailing address: Department of General and Environmental Physiology, University Federico II, via Mezzocannone 16, 80134 Naples, Italy. Phone: 39-081-5514299. Fax: 39-081-5514437. E-mail: ericca{at}unina.it.

dagger Present address: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2700 Oeiras, Portugal.

Journal of Bacteriology, April 1999, p. 2631-2633, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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