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Journal of Bacteriology, April 1999, p. 2634-2639, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Differential Regulation of Two Divergent Sinorhizobium meliloti Genes for HPII-Like Catalases during Free-Living Growth and Protective Role of Both Catalases during Symbiosis

Samuel Sigaud, Vanessa Becquet, Pierre Frendo, Alain Puppo, and Didier Hérouart*

Laboratoire de Biologie Végétale et Microbiologie, Unité de Recherche Associée ERS 590, Centre National de la Recherche Scientifique, Université de Nice Sophia-Antipolis, 06108 Nice Cedex 2, France

Received 22 October 1998/Accepted 2 February 1999

Two catalases, KatA and KatB, have been detected in Sinorhizobium meliloti growing on rich medium. Here we characterize a new catalase gene encoding a third catalase (KatC). KatC activity was detectable only at the end of the stationary phase in S. meliloti growing in minimum medium, whereas KatA activity was found during the exponential phase. Analysis with a katC-lacZ fusion demonstrated that katC expression is mainly regulated at the transcription level. An increase of catalase activity correlating with KatA induction was detected in bacteroids. A dramatic decrease of nitrogen fixation capacity in a katA katC double mutant was observed, suggesting that these catalases are very important for the protection of the nitrogen fixation process.

* Corresponding author. Mailing address: Laboratoire de Biologie Végétale et Microbiologie, ERS CNRS 590, Université de Nice Sophia-Antipolis, Parc Valrose, 06108, Nice Cedex 2, France. Phone: (33) 492076551. Fax: (33) 492076838. E-mail: herouart{at}unice.fr.

Journal of Bacteriology, April 1999, p. 2634-2639, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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