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Journal of Bacteriology, April 1999, p. 2652-2654, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Type 2 Capsule Locus of Streptococcus pneumoniae

Francesco Iannelli, Barbara J. Pearce,dagger and Gianni Pozzi*

Sezione di Microbiologia, Dipartimento di Biologia Molecolare, Università di Siena, 53100 Siena, Italy

Received 12 August 1998/Accepted 29 January 1999

The type 2 capsule locus of Streptococcus pneumoniae was characterized in Avery's strain D39, which is the parent strain of the standard transformation recipients currently used in pneumococcal research and is largely used as a virulent strain in studies on the pathogenesis of pneumococcal infections. The capsule locus was sequenced by using a 21.7-kb PCR fragment from the D39 genome as a template. Sequence data analysis showed the presence of 18 open reading frames, 17 of which have the same direction of transcription and all of which are potentially involved in capsule biosynthesis. It was also shown that R36A and R6, which are unencapsulated (rough) derivatives of D39, carry a 7,504-bp deletion involving nine capsule genes.

* Corresponding author. Mailing address: Microbiologia/Università di Siena, Via Laterina 8, 53100 Siena, Italy. Phone: 39-0577-233874. Fax: 39-0577-233870. E-mail: pozzi{at}unisi.it.

dagger Present address: 16/19 Flinders Rd., Earlwood, NSW 2206, Australia.

Journal of Bacteriology, April 1999, p. 2652-2654, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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