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Journal of Bacteriology, April 1999, p. 2655-2658, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Azorhizobium caulinodans PII and GlnK Proteins Control Nitrogen Fixation and Ammonia Assimilation

Nathalie Michel-Reydelletdagger and P. Alexandre Kaminski*

Unité de Physiologie Cellulaire, Centre National de la Recherche Scientifique, Unité Recherche Associée 1300, Département des Biotechnologies, Institut Pasteur, 75724 Paris Cedex 15, France

Received 15 October 1998/Accepted 5 February 1999

We herein report that Azorhizobium caulinodans PII and GlnK are not necessary for glutamine synthetase (GS) adenylylation whereas both proteins are required for complete GS deadenylylation. The disruption of both glnB and glnK resulted in a high level of GS adenylylation under the condition of nitrogen fixation, leading to ammonium excretion in the free-living state. PII and GlnK also controlled nif gene expression because NifA activated nifH transcription and nitrogenase activity was derepressed in glnB glnK double mutants, but not in wild-type bacteria, grown in the presence of ammonia.

* Corresponding author. Mailing address: Unité de Biochimie Cellulaire, Département de Biochimie et Génétique Moléculaire, Institut Pasteur, 28 rue de Dr. Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 45 68 83 88. Fax: (33)1 40 61 30 43. E-mail: akaminsk{at}pasteur.fr.

dagger Present address: Laboratory of Biochemical Engineering, Department of Chemical Engineering, Stanford University, Stanford, CA 94305-5025. 

Journal of Bacteriology, April 1999, p. 2655-2658, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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