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Journal of Bacteriology, May 1999, p. 2802-2806, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Periplasmic Nitrate Reductase in Pseudomonas sp. Strain G-179 Catalyzes the First Step of Denitrification

Laura Bedzyk, Tao Wang, and Rick W. Ye*

DuPont Central Research and Development, Wilmington, Delaware 19880-0328

Received 23 October 1998/Accepted 26 February 1999

Both membrane-bound and periplasmic nitrate reductases have been found in denitrifying bacteria. Yet the role of periplasmic nitrate reductase in denitrification has not been clearly defined. To analyze the function of the periplasmic nitrate reductase in Pseudomonas sp. strain G-179, the nap gene cluster was identified and found to be linked to genes involved in reduction of nitrite and nitric oxide and anaerobic heme biosynthesis. Mutation in the nap region rendered the cells incapable of growing under anaerobic conditions with nitrate as the alternative electron acceptor. No nitrate reduction activity was detected in the Nap- mutant, but that activity could be restored by complementation with the nap region. Unlike the membrane-bound nitrate reductase, the nitrate reduction activity in strain G-179 was not inhibited by a low concentration of azide. Nor could it use NADH as the electron donor to reduce nitrate or use chlorate as the alternative substrate. These results suggest that the periplasmic nitrate reductase in this strain plays a primary role in dissimilatory nitrate reduction.

* Corresponding author. Mailing address: Experimental Station E328/148B, Route 141 and Henry Clay Rd., Wilmington, DE 19880-0328. Phone: (302) 695-1750. Fax: (302) 695-1829. E-mail: rick.ye{at}usa.dupont.com.

Journal of Bacteriology, May 1999, p. 2802-2806, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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