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Journal of Bacteriology, May 1999, p. 2938-2941, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Histidine Operon Deattenuation in dnaA Mutants of Salmonella typhimurium Correlates with a Decrease in the Gene Dosage Ratio between tRNAHis and Histidine Biosynthetic Loci

Anne-Beatrice Blanc-Potard,dagger Nara Figueroa-Bossi, and Lionello Bossi*

Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France

Received 18 November 1998/Accepted 17 February 1999

Expression of the histidine operon of Salmonella typhimurium is increased in dnaA(Ts) mutants at 37°C. This effect requires an intact his attenuator and can be suppressed by increasing the gene copy number of the hisR locus, which encodes the tRNAHis. We present data which suggest that the his deattenuation defect in dnaA(Ts) mutants results from the loss of a gene dosage gradient between the hisR locus, close to oriC, and the his operon, far from oriC. Some of the conclusions drawn here may apply to other operons as well.

* Corresponding author. Mailing address: Centre de Génétique Moléculaire, CNRS, 91198 Gif-sur-Yvette Cedex, France. Phone: 33 1 69 82 31 37. Fax: 33 1 69 82 32 30. E-mail: Bossi{at}cgm.cnrs-gif.fr.

dagger Present address: INSERM U.510, Faculté de Pharmacie Paris XI, 92296 Châtenay-Malabry Cedex, France.

Journal of Bacteriology, May 1999, p. 2938-2941, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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