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Journal of Bacteriology, January 2000, p. 211-215, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Uptake of 2-Oxoglutarate in Synechococcus Strains Transformed with the Escherichia coli kgtP Gene

María Félix Vázquez-Bermúdez, Antonia Herrero, and Enrique Flores*

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, E-41092 Seville, Spain

Received 7 June 1999/Accepted 14 October 1999

A number of cyanobacteria from different taxonomic groups exhibited very low levels of uptake of 2-[U-14C]oxoglutarate. Synechococcus sp. strain PCC 7942 was transformed with DNA constructs carrying the Escherichia coli kgtP gene encoding a 2-oxoglutarate permease and a kanamycin resistance gene cassette. The Synechococcus sp. strains bearing the kgtP gene incorporated 2-oxoglutarate into the cells through an active transport process. About 75% of the radioactivity from the 2-[U-14C]oxoglutarate taken up that was recovered in soluble metabolites was found as glutamate and glutamine. 2-Oxoglutarate was, however, detrimental to the growth of a Synechococcus sp. strain bearing the kgtP gene.

* Corresponding author. Mailing address: Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Avda. Américo Vespucio s/n, E-41092 Seville, Spain. Phone: 34 95 448 9523. Fax: 34 95 446 0065. E-mail: flores{at}cica.es.

Journal of Bacteriology, January 2000, p. 211-215, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Li, J.-H., Laurent, S., Konde, V., Bedu, S., Zhang, C.-C. (2003). An increase in the level of 2-oxoglutarate promotes heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Microbiology 149: 3257-3263 [Abstract] [Full Text]  
  • Herrero, A., Muro-Pastor, A. M., Flores, E. (2001). Nitrogen Control in Cyanobacteria. J. Bacteriol. 183: 411-425 [Full Text]  


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