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Journal of Bacteriology, January 2000, p. 221-224, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Effects of Amino Acid Substitutions at Conserved and Acidic Residues within Region 1.1 of Escherichia coli sigma 70

Christina Wilson Bowers, Andrea McCracken, and Alicia J. Dombroski*

Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Houston, Texas 77030

Received 14 September 1999/Accepted 6 October 1999

Amino acid substitutions in Escherichia coli sigma 70 were generated and characterized in an analysis of the role of region 1.1 in transcription initiation. Several acidic and conserved residues are tolerant of substitution. However, replacement of aspartic acid 61 with alanine results in inactivity caused by structural and functional thermolability.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, 6431 Fannin JFB1.765, Houston, TX 77030. Phone: (713) 500-5442. Fax: (713) 500-5499. E-mail: dombros{at}utmmg.med.uth.tmc.edu.

Journal of Bacteriology, January 2000, p. 221-224, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gowrishankar, J., Yamamoto, K., Subbarayan, P. R., Ishihama, A. (2003). In Vitro Properties of RpoS ({sigma}S) Mutants of Escherichia coli with Postulated N-Terminal Subregion 1.1 or C-Terminal Region 4 Deleted. J. Bacteriol. 185: 2673-2679 [Abstract] [Full Text]  
  • Givens, J. R., McGovern, C. L., Dombroski, A. J. (2001). Formation of Intermediate Transcription Initiation Complexes at pfliD and pflgM by {sigma}28 RNA Polymerase. J. Bacteriol. 183: 6244-6252 [Abstract] [Full Text]  
  • Southern, E., Merrick, M. (2000). The role of Region II in the RNA polymerase {sigma} factor {sigma}N ({sigma}54). Nucleic Acids Res 28: 2563-2570 [Abstract] [Full Text]  


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