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Journal of Bacteriology, May 2000, p. 2680-2686, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Assessment of flhDC mRNA Levels in Serratia liquefaciens Swarm Cells

Tim Tolker-Nielsen,1 Allan Beck Christensen,1 Kim Holmstrøm,2 Leo Eberl,3 Thomas Bovbjerg Rasmussen,1 Claus Sternberg,1 Arne Heydorn,1 Søren Molin,1 and Michael Givskov1,*

Department of Microbiology, The Technical University of Denmark, DK-2800 Lyngby,1 and Biotechnological Institute, DK-2970 Hørsholm,2 Denmark, and Lehrstuhl für Mikrobiologie, Technische Universität München, D-80290 Munich, Germany3

Received 11 November 1999/Accepted 24 February 2000

We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.

* Corresponding author. Mailing address: Department of Microbiology, DTU, Building 301, DK-2800 Lyngby, Denmark. Phone: 45 45252769. Fax: 45 45932809. E-mail: immg{at}pop.dtu.dk.

Journal of Bacteriology, May 2000, p. 2680-2686, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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