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Journal of Bacteriology, May 2000, p. 2970-2972, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Complete Nucleotide Sequence of Tn10

Ronald Chalmers,* Sven Sewitz, Karen Lipkow, and Paul Crellin

Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom

Received 7 September 1999/Accepted 4 February 2000

The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein in Bacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protect Escherichia coli from the toxic effects of various metals.


* Corresponding author. Mailing address: Department of Biochemistry, University of Oxford, South Parks Rd., Oxford OX1 3QU, United Kingdom. Phone: 44-1865-275307. Fax: 44-1865-275297. E-mail: chalmers{at}bioch.ox.ac.uk.


Journal of Bacteriology, May 2000, p. 2970-2972, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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