This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, L. W.
Right arrow Articles by Schneewind, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, L. W.
Right arrow Articles by Schneewind, O.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2000, p. 3183-3190, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Yersinia enterocolitica TyeA, an Intracellular Regulator of the Type III Machinery, Is Required for Specific Targeting of YopE, YopH, YopM, and YopN into the Cytosol of Eukaryotic Cells

Luisa W. Cheng and Olaf Schneewind*

Department of Microbiology and Immunology, University of California---Los Angeles School of Medicine, Los Angeles, California 90095

Received 9 December 1999/Accepted 16 March 2000

Pathogenic Yersinia species employ type III machines to target effector Yops into the cytosol of eukaryotic cells. Yersinia tyeA mutants are thought to be defective in the targeting of YopE and YopH without affecting the injection of YopM, YopN, YopO, YopP, and YopT into the cytosol of eukaryotic cells. One model suggests that TyeA may form a tether between YopN (LcrE) and YopD on the bacterial surface, a structure that may translocate YopE and YopH across the plasma membrane of eukaryotic cells (M. Iriarte, M. P. Sory, A. Boland, A. P. Boyd, S. D. Mills, I. Lambermont, and G. R. Cornelis, EMBO J. 17:1907-1918, 1998). We have examined the injection of Yop proteins by tyeA mutant yersiniae with the digitonin fractionation technique. We find that tyeA mutant yersiniae not only secreted YopE, YopH, YopM, and YopN into the extracellular medium but also targeted these polypeptides into the cytosol of HeLa cells. Protease protection, cell fractionation, and affinity purification experiments suggest that TyeA is located intracellularly and binds to YopN or YopD. We propose a model whereby TyeA functions as a negative regulator of the type III targeting pathway in the cytoplasm of yersiniae, presumably by preventing the export of YopN.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, UCLA School of Medicine, 10833 Le Conte Ave., Los Angeles, CA 90095. Phone: (310) 206-0997. Fax: (310) 267-0173. E-mail: olafs{at}ucla.edu.

Journal of Bacteriology, June 2000, p. 3183-3190, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Riordan, K. E., Sorg, J. A., Berube, B. J., Schneewind, O. (2008). Impassable YscP Substrates and Their Impact on the Yersinia enterocolitica Type III Secretion Pathway. J. Bacteriol. 190: 6204-6216 [Abstract] [Full Text]  
  • Falker, S., Schmidt, M. A., Heusipp, G. (2006). Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG.. J. Bacteriol. 188: 7072-7081 [Abstract] [Full Text]  
  • Davis, J. M., Carvalho, H. M., Rasmussen, S. B., O'Brien, A. D. (2006). Cytotoxic Necrotizing Factor Type 1 Delivered by Outer Membrane Vesicles of Uropathogenic Escherichia coli Attenuates Polymorphonuclear Leukocyte Antimicrobial Activity and Chemotaxis.. Infect. Immun. 74: 4401-4408 [Abstract] [Full Text]  
  • Heusipp, G., Spekker, K., Brast, S., Falker, S., Schmidt, M. A. (2006). YopM of Yersinia enterocolitica specifically interacts with {alpha}1-antitrypsin without affecting the anti-protease activity.. Microbiology 152: 1327-1335 [Abstract] [Full Text]  
  • Kim, J., Ahn, K., Min, S., Jia, J., Ha, U., Wu, D., Jin, S. (2005). Factors triggering type III secretion in Pseudomonas aeruginosa. Microbiology 151: 3575-3587 [Abstract] [Full Text]  
  • Tautz, L., Bruckner, S., Sareth, S., Alonso, A., Bogetz, J., Bottini, N., Pellecchia, M., Mustelin, T. (2005). Inhibition of Yersinia Tyrosine Phosphatase by Furanyl Salicylate Compounds. J. Biol. Chem. 280: 9400-9408 [Abstract] [Full Text]  
  • Goss, J. W., Sorg, J. A., Ramamurthi, K. S., Ton-That, H., Schneewind, O. (2004). The Secretion Signal of YopN, a Regulatory Protein of the Yersinia enterocolitica Type III Secretion Pathway. J. Bacteriol. 186: 6320-6324 [Abstract] [Full Text]  
  • Ferracci, F., Day, J. B., Ezelle, H. J., Plano, G. V. (2004). Expression of a Functional Secreted YopN-TyeA Hybrid Protein in Yersinia pestis Is the Result of a +1 Translational Frameshift Event. J. Bacteriol. 186: 5160-5166 [Abstract] [Full Text]  
  • Alonso, A., Bottini, N., Bruckner, S., Rahmouni, S., Williams, S., Schoenberger, S. P., Mustelin, T. (2004). Lck Dephosphorylation at Tyr-394 and Inhibition of T Cell Antigen Receptor Signaling by Yersinia Phosphatase YopH. J. Biol. Chem. 279: 4922-4928 [Abstract] [Full Text]  
  • Broms, J. E., Forslund, A.-L., Forsberg, A., Francis, M. S. (2003). Dissection of homologous translocon operons reveals a distinct role for YopD in type III secretion by Yersinia pseudotuberculosis. Microbiology 149: 2615-2626 [Abstract] [Full Text]  
  • DeBord, K. L., Galanopoulos, N. S., Schneewind, O. (2003). The ttsA Gene Is Required for Low-Calcium-Induced Type III Secretion of Yop Proteins and Virulence of Yersinia enterocolitica W22703. J. Bacteriol. 185: 3499-3507 [Abstract] [Full Text]  
  • Mavris, M., Sansonetti, P. J., Parsot, C. (2002). Identification of the cis-Acting Site Involved in Activation of Promoters Regulated by Activity of the Type III Secretion Apparatus in Shigella flexneri. J. Bacteriol. 184: 6751-6759 [Abstract] [Full Text]  
  • Cambronne, E. D., Schneewind, O. (2002). Yersinia enterocolitica Type III Secretion: yscM1 and yscM2 Regulate yop Gene Expression by a Posttranscriptional Mechanism That Targets the 5' Untranslated Region of yop mRNA. J. Bacteriol. 184: 5880-5893 [Abstract] [Full Text]  
  • Kubori, T., Galan, J. E. (2002). Salmonella Type III Secretion-Associated Protein InvE Controls Translocation of Effector Proteins into Host Cells. J. Bacteriol. 184: 4699-4708 [Abstract] [Full Text]  
  • Cornelis, G. R. (2002). Yersinia type III secretion: send in the effectors. JCB 158: 401-408 [Abstract] [Full Text]  
  • Ramamurthi, K. S., Schneewind, O. (2002). Yersinia enterocolitica Type III Secretion: Mutational Analysis of the yopQ Secretion Signal. J. Bacteriol. 184: 3321-3328 [Abstract] [Full Text]  
  • Anderson, D. M., Ramamurthi, K. S., Tam, C., Schneewind, O. (2002). YopD and LcrH Regulate Expression of Yersinia enterocolitica YopQ by a Posttranscriptional Mechanism and Bind to yopQ RNA. J. Bacteriol. 184: 1287-1295 [Abstract] [Full Text]  
  • Montigiani, S., Falugi, F., Scarselli, M., Finco, O., Petracca, R., Galli, G., Mariani, M., Manetti, R., Agnusdei, M., Cevenini, R., Donati, M., Nogarotto, R., Norais, N., Garaguso, I., Nuti, S., Saletti, G., Rosa, D., Ratti, G., Grandi, G. (2002). Genomic Approach for Analysis of Surface Proteins in Chlamydia pneumoniae. Infect. Immun. 70: 368-379 [Abstract] [Full Text]  
  • Cheng, L. W., Kay, O., Schneewind, O. (2001). Regulated Secretion of YopN by the Type III Machinery of Yersinia enterocolitica. J. Bacteriol. 183: 5293-5301 [Abstract] [Full Text]  
  • Lee, V. T., Mazmanian, S. K., Schneewind, O. (2001). A Program of Yersinia enterocolitica Type III Secretion Reactions Is Activated by Specific Signals. J. Bacteriol. 183: 4970-4978 [Abstract] [Full Text]  
  • Matson, J. S., Nilles, M. L. (2001). LcrG-LcrV Interaction Is Required for Control of Yops Secretion in Yersinia pestis. J. Bacteriol. 183: 5082-5091 [Abstract] [Full Text]  
  • DeBord, K. L., Lee, V. T., Schneewind, O. (2001). Roles of LcrG and LcrV during Type III Targeting of Effector Yops by Yersinia enterocolitica. J. Bacteriol. 183: 4588-4598 [Abstract] [Full Text]  
  • Nordfelth, R., Wolf-Watz, H. (2001). YopB of Yersinia enterocolitica Is Essential for YopE Translocation. Infect. Immun. 69: 3516-3518 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»