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Journal of Bacteriology, June 2000, p. 3204-3209, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Another Unusual Type of Citric Acid Cycle Enzyme in Helicobacter pylori: the Malate:Quinone Oxidoreductase

Birgit Kather,1 Kerstin Stingl,2 Michel E. van der Rest,1 Karlheinz Altendorf,2 and Douwe Molenaar1,*

Biotechnologisches Zentrallabor, Geb. 25.12, Heinrich-Heine-Universität, D-40225 Düsseldorf,1 and Abteilung Mikrobiologie, Universität Osnabrück, D-49076 Osnabrück,2 Germany

Received 14 January 2000/Accepted 19 March 2000

The only enzyme of the citric acid cycle for which no open reading frame (ORF) was found in the Helicobacter pylori genome is the NAD-dependent malate dehydrogenase. Here, it is shown that in this organism the oxidation of malate to oxaloacetate is catalyzed by a malate:quinone oxidoreductase (MQO). This flavin adenine dinucleotide-dependent membrane-associated enzyme donates electrons to quinones of the electron transfer chain. Similar to succinate dehydrogenase, it is part of both the electron transfer chain and the citric acid cycle. MQO activity was demonstrated in isolated membranes of H. pylori. The enzyme is encoded by the ORF HP0086, which is shown by the fact that expression of the HP0086 sequence from a plasmid induces high MQO activity in mqo deletion mutants of Escherichia coli or Corynebacterium glutamicum. Furthermore, this plasmid was able to complement the phenotype of the C. glutamicum mqo deletion mutant. Interestingly, the protein predicted to be encoded by this ORF is only distantly related to known or postulated MQO sequences from other bacteria. The presence of an MQO shown here and the previously demonstrated presence of a 2-ketoglutarate:ferredoxin oxidoreductase and a succinyl-coenzyme A (CoA):acetoacetyl-CoA transferase indicate that H. pylori possesses a complete citric acid cycle, but one which deviates from the standard textbook example in three steps.

* Corresponding author. Mailing address: Biotechnologisches Zentrallabor, Geb. 25.12, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225 Düsseldorf, Germany. Phone: 49 211 811 1482. Fax: 49 211 811 5370. E-mail: molenaar{at}rz.uni-duesseldorf.de.

Journal of Bacteriology, June 2000, p. 3204-3209, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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