This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Letley, D. P.
Right arrow Articles by Atherton, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Letley, D. P.
Right arrow Articles by Atherton, J. C.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2000, p. 3278-3280, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Natural Diversity in the N Terminus of the Mature Vacuolating Cytotoxin of Helicobacter pylori Determines Cytotoxin Activity

D. P. Letley* and J. C. Atherton

Division of Gastroenterology and Institute of Infections and Immunity, University of Nottingham, Nottingham NG7 2UH, United Kingdom

Received 26 October 1999/Accepted 7 March 2000

Naturally occurring noncytotoxic vacA type s2 strains of Helicobacter pylori have a 12-residue extension to the vacuolating cytotoxin (VacA) compared with cytotoxic type s1 strains. We show that adding the region encoding this extension to type s1 vacA completely abolishes vacuolating cytotoxin activity but has no effect on VacA production.

* Corresponding author. Mailing address: Division of Gastroenterology, Queen's Medical Centre, University Hospital, Clifton Blvd., Nottingham NG7 2UH, United Kingdom. Phone: (44) 115-9249924. Fax: (44) 115-9709923. E-mail: Darren.Letley{at}nottingham.ac.uk.

Journal of Bacteriology, June 2000, p. 3278-3280, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ogiwara, H., Sugimoto, M., Ohno, T., Vilaichone, R.-K., Mahachai, V., Graham, D. Y., Yamaoka, Y. (2009). Role of Deletion Located between the Intermediate and Middle Regions of the Helicobacter pylori vacA Gene in Cases of Gastroduodenal Diseases. J. Clin. Microbiol. 47: 3493-3500 [Abstract] [Full Text]  
  • Sheu, S.-M., Hung, K.-H., Sheu, B.-S., Yang, H.-B., Wu, J.-J. (2009). Association of Nonsynonymous Substitutions in the Intermediate Region of the vacA Gene of Helicobacter pylori with Gastric Diseases in Taiwan. J. Clin. Microbiol. 47: 249-251 [Abstract] [Full Text]  
  • Owen, R. J., Xerry, J. (2007). Geographical conservation of short inserts in the signal and middle regions of the Helicobacter pylori vacuolating cytotoxin gene. Microbiology 153: 1176-1186 [Abstract] [Full Text]  
  • McClain, M. S., Czajkowsky, D. M., Torres, V. J., Szabo, G., Shao, Z., Cover, T. L. (2006). Random Mutagenesis of Helicobacter pylori vacA To Identify Amino Acids Essential for Vacuolating Cytotoxic Activity. Infect. Immun. 74: 6188-6195 [Abstract] [Full Text]  
  • Fitchen, N., Letley, D. P, O'Shea, P., Atherton, J. C, Williams, P., Hardie, K. R (2005). All subtypes of the cytotoxin VacA adsorb to the surface of Helicobacter pylori post-secretion. J Med Microbiol 54: 621-630 [Abstract] [Full Text]  
  • Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  
  • Aviles-Jimenez, F., Letley, D. P., Gonzalez-Valencia, G., Salama, N., Torres, J., Atherton, J. C. (2004). Evolution of the Helicobacter pylori Vacuolating Cytotoxin in a Human Stomach. J. Bacteriol. 186: 5182-5185 [Abstract] [Full Text]  
  • Letley, D. P., Rhead, J. L., Twells, R. J., Dove, B., Atherton, J. C. (2003). Determinants of Non-toxicity in the Gastric Pathogen Helicobacter pylori. J. Biol. Chem. 278: 26734-26741 [Abstract] [Full Text]  
  • Cover, T. L., Krishna, U. S., Israel, D. A., Peek, R. M. Jr. (2003). Induction of Gastric Epithelial Cell Apoptosis by Helicobacter pylori Vacuolating Cytotoxin. Cancer Res. 63: 951-957 [Abstract] [Full Text]  
  • Cao, P., Cover, T. L. (2002). Two Different Families of hopQ Alleles in Helicobacter pylori. J. Clin. Microbiol. 40: 4504-4511 [Abstract] [Full Text]  
  • Kalia, N, Bardhan, K D, Atherton, J C, Brown, N J (2002). Toxigenic Helicobacter pylori induces changes in the gastric mucosal microcirculation in rats. Gut 51: 641-647 [Abstract] [Full Text]  
  • McClain, M. S., Cao, P., Iwamoto, H., Vinion-Dubiel, A. D., Szabo, G., Shao, Z., Cover, T. L. (2001). A 12-Amino-Acid Segment, Present in Type s2 but Not Type s1 Helicobacter pylori VacA Proteins, Abolishes Cytotoxin Activity and Alters Membrane Channel Formation. J. Bacteriol. 183: 6499-6508 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»