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Journal of Bacteriology, June 2000, p. 3298-3301, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Secretion of the Caulobacter crescentus S-Layer Protein: Further Localization of the C-Terminal Secretion Signal and Its Use for Secretion of Recombinant Proteins

Wade H. Bingle, John F. Nomellini, and John Smit*

Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada

Received 17 September 1999/Accepted 15 March 2000

The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) was localized to the C-terminal 82 amino acids of the molecule. Protein yield studies showed that 336 or 242 C-terminal residues of RsaA mediated secretion of >50 mg of a cellulase passenger protein per liter to the culture fluids.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of British Columbia, 300-6174 University Blvd., Vancouver, British Columbia, Canada V6T 1Z3. Phone: (604) 822-4417. Fax: (604) 822-6041. E-mail: jsmit{at}interchange.ubc.ca.

Journal of Bacteriology, June 2000, p. 3298-3301, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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