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Journal of Bacteriology, June 2000, p. 3416-3422, Vol. 182, No. 12
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

In Lactobacillus plantarum, Carbamoyl Phosphate Is Synthesized by Two Carbamoyl-Phosphate Synthetases (CPS): Carbon Dioxide Differentiates the Arginine-Repressed from the Pyrimidine-Regulated CPS

Hervé Nicoloff, Jean-Claude Hubert, and Françoise Bringel*

Laboratoire de Microbiologie et de Génétique, Université Louis-Pasteur, CNRS UPRES A7010, Strasbourg, France

Received 19 January 2000/Accepted 22 February 2000

Carbamoyl phosphate (CP) is an intermediate in pyrimidine and arginine biosynthesis. Carbamoyl-phosphate synthetase (CPS) contains a small amidotransferase subunit (GLN) that hydrolyzes glutamine and transfers ammonia to the large synthetase subunit (SYN), where CP biosynthesis occurs in the presence of ATP and CO2. Lactobacillus plantarum, a lactic acid bacterium, harbors a pyrimidine-inhibited CPS (CPS-P; Elagöz et al., Gene 182:37-43, 1996) and an arginine-repressed CPS (CPS-A). Sequencing has shown that CPS-A is encoded by carA (GLN) and carB (SYN). Transcriptional studies have demonstrated that carB is transcribed both monocistronically and in the carAB arginine-repressed operon. CP biosynthesis in L. plantarum was studied with three mutants (Delta CPS-P, Delta CPS-A, and double deletion). In the absence of both CPSs, auxotrophy for pyrimidines and arginine was observed. CPS-P produced enough CP for both pathways. In CO2-enriched air but not in ordinary air, CPS-A provided CP only for arginine biosynthesis. Therefore, the uracil sensitivity observed in prototrophic wild-type L. plantarum without CO2 enrichment may be due to the low affinity of CPS-A for its substrate CO2 or to regulation of the CP pool by the cellular CO2/bicarbonate level.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et de Génétique, Université Louis-Pasteur, CNRS UPRES A7010, 28 rue Goethe F-67083 Strasbourg, France. Phone: (33) 3 88 24 41 53. Fax: (33) 3 88 35 84 84. E-mail: bringel{at}gem.u-strasbg.fr.

Journal of Bacteriology, June 2000, p. 3416-3422, Vol. 182, No. 12
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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