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Journal of Bacteriology, June 2000, p. 3602-3606, Vol. 182, No. 12
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

First Evidence for Existence of an Uphill Electron Transfer through the bc1 and NADH-Q Oxidoreductase Complexes of the Acidophilic Obligate Chemolithotrophic Ferrous Ion-Oxidizing Bacterium Thiobacillus ferrooxidans

A. Elbehti,dagger G. Brasseur, and D. Lemesle-Meunier*

Bioénergétique et Ingénierie des Proteines, CNRS, Institut de Biologie Structurale et Microbiologie, 13402 Marseille, cedex 20, France

Received 18 February 2000/Accepted 29 March 2000

The energy-dependent electron transfer pathway involved in the reduction of pyridine nucleotides which is required for CO2 fixation to occur in the acidophilic chemolithotrophic organism Thiobacillus ferrooxidans was investigated using ferrocytochrome c as the electron donor. The experimental results show that this uphill pathway involves a bc1 and an NADH-Q oxidoreductase complex functioning in reverse, using an electrochemical proton gradient generated by ATP hydrolysis. Based on these results, a model is presented to explain the balance of the reducing equivalent from ferrocytochrome c between the exergonic and endergonic electron transfer pathways.

* Corresponding author. Mailing address: Bioénergétique et Ingénierie des Proteins, CNRS, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, 13402 Marseille, cedex 20, France. Phone: 33 4 91 16 44 86. Fax: 33 4 91 16 45 63. E-mail: lemesle{at}ibsm.cnrs-mrs.fr.

dagger Present address: Department of Medicine and Cancer Center, University of California, San Diego, La Jolla, CA 92093-0688.

Journal of Bacteriology, June 2000, p. 3602-3606, Vol. 182, No. 12
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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