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Journal of Bacteriology, July 2000, p. 3839-3842, Vol. 182, No. 13
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Synechocystis Strain PCC 6803 cya2, a Prokaryotic Gene That Encodes a Guanylyl Cyclase

Jesús A. G. Ochoa de Alda,1 Ghada Ajlani,2 and Jean Houmard1,*

Dynamique des Membranes Végétales, Complexes Protéines-Pigments, CNRS UMR8543, Ecole Normale Supérieure, 75230 Paris Cedex 05, France,1 and CNRS URA2096, DBCM/SBPM, CEA Saclay, 91191 Gif sur Yvette Cedex, France2

Received 1 February 2000/Accepted 30 March 2000

Synechocystis strain PCC 6803 exhibits similar levels of cyclic AMP (cAMP) and cyclic GMP (cGMP). A thorough analysis of its genome showed that Cya2 (Sll0646) has all the sequence determinants required in terms of activity and purine specificity for being a guanylyl cyclase. Insertional mutagenesis of cya2 caused a marked reduction in cGMP content without altering the cAMP content. Thus, Cya2 represents the first example of a prokaryotic guanylyl cyclase.

* Corresponding author. Mailing address: Dynamique des Membranes Végétales, Complexes Protéines-Pigments, CNRS UMR8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France. Phone: 33 1 44 32 35 19. Fax: 33 1 44 32 39 35. E-mail: jhoumard{at}biologie.ens.fr.

Journal of Bacteriology, July 2000, p. 3839-3842, Vol. 182, No. 13
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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