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Journal of Bacteriology, July 2000, p. 4101-4103, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning of the Gene Coding for Staphylococcus hyicus Exfoliative Toxin B and Its Expression in Escherichia coli

Takao Watanabe,1 Hisaaki Sato,1,* Yu Hatakeyama,1 Takeshi Matsuzawa,1 Masanori Kawai,1 Chikara Aizawa,2 Hirofumi Danbara,3 and Nobutoshi Maehara1

Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628,1 and The Kitasato Institute2 and Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University,3 Shirokane, Minato-ku, Tokyo 108-0072, Japan

Received 14 October 1998/Accepted 24 April 2000

The Staphylococcus hyicus exfoliative toxin B (SHETB) gene was cloned into pUC118 and expressed in Escherichia coli. The nucleotide sequence of the SHETB gene consists of a coding region of 804 bp specifying a polypeptide of 268 amino acid residues, which included a putative 20-residue signal sequence.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan. Phone: 81-176-23-4371. Fax: 81-176-23-8703. E-mail: satoh{at}vmas.kitasato-u.ac.jp.

Journal of Bacteriology, July 2000, p. 4101-4103, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ahrens, P., Andresen, L. O. (2004). Cloning and Sequence Analysis of Genes Encoding Staphylococcus hyicus Exfoliative Toxin Types A, B, C, and D. J. Bacteriol. 186: 1833-1837 [Abstract] [Full Text]  
  • Yamaguchi, T., Nishifuji, K., Sasaki, M., Fudaba, Y., Aepfelbacher, M., Takata, T., Ohara, M., Komatsuzawa, H., Amagai, M., Sugai, M. (2002). Identification of the Staphylococcus aureus etd Pathogenicity Island Which Encodes a Novel Exfoliative Toxin, ETD, and EDIN-B. Infect. Immun. 70: 5835-5845 [Abstract] [Full Text]  


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