This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mukhopadhyaya, P. N.
Right arrow Articles by Roy, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mukhopadhyaya, P. N.
Right arrow Articles by Roy, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2000, p. 4278-4287, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A soxA Gene, Encoding a Diheme Cytochrome c, and a sox Locus, Essential for Sulfur Oxidation in a New Sulfur Lithotrophic Bacterium

Pratap N. Mukhopadhyaya,dagger Chirajyoti Deb,Dagger Chandrajit Lahiri, and Pradosh Roy*

Department of Microbiology, Bose Institute, P-1/12, C. I. T. Scheme VII-M, Calcutta-700 054, India

Received 18 October 1999/Accepted 4 May 2000

A mobilizable suicide vector, pSUP5011, was used to introduce Tn5-mob in a new facultative sulfur lithotrophic bacterium, KCT001, to generate mutants defective in sulfur oxidation (Sox-). The Sox- mutants were unable to oxidize thiosulfate while grown mixotrophically in the presence of thiosulfate and succinate. The mutants were also impaired in oxidizing other reduced sulfur compounds and elemental sulfur as evident from the study of substrate oxidation by the whole cells. Sulfite oxidase activity was significantly diminished in the cell extracts of all the mutants. A soxA gene was identified from the transposon-adjacent genomic DNA of a Sox- mutant strain. The sequence analysis revealed that the soxA open reading frame (ORF) is preceded by a potential ribosome binding site and promoter region with -10- and -35-like sequences. The deduced nucleotide sequence of the soxA gene was predicted to code for a protein of 286 amino acids. It had a signal peptide of 26 N-terminal amino acids. The amino acid sequence showed similarity with a putative gene product of Aquifex aeolicus, soluble cytochrome c551 of Chlorobium limicola, and the available partial SoxA sequence of Paracoccus denitrificans. The soxA-encoded product seems to be a diheme cytochrome c for KCT001 and A. aeolicus, but the amino acid sequence of C. limicola cytochrome c551 revealed a single heme-binding region. Another transposon insertion mutation was mapped within the soxA ORF. Four other independent transposon insertion mutations were mapped in the 4.4-kb soxA contiguous genomic DNA region. The results thus suggest that a sox locus of KCT001, essential for sulfur oxidation, was affected by all these six independent insertion mutations.

* Corresponding author. Mailing address: Department of Microbiology, Bose Institute, P-1/12, C. I. T. Scheme VII-M, Calcutta-700 054, India. Phone: 91-033-334-7430. Fax: 91-033-334-3886. E-mail: prodosh{at}boseinst.ernet.in.

dagger Present address: Molecular Genetics Laboratory, Biotechnology Group, Research and Development Division, National Dairy Development Board, Anand-388001, India.

Dagger Present address: Molecular Genetics Laboratory, Indian Institute of Chemical Biology, Calcutta-700 032, India.

Journal of Bacteriology, August 2000, p. 4278-4287, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sauve, V., Roversi, P., Leath, K. J., Garman, E. F., Antrobus, R., Lea, S. M., Berks, B. C. (2009). Mechanism for the Hydrolysis of a Sulfur-Sulfur Bond Based on the Crystal Structure of the Thiosulfohydrolase SoxB. J. Biol. Chem. 284: 21707-21718 [Abstract] [Full Text]  
  • Yoo, S.-H., Weon, H.-Y., Anandham, R., Kim, B.-Y., Hong, S.-B., Jeon, Y.-A., Koo, B.-S., Kwon, S.-W. (2009). Dokdonella soli sp. nov., a gammaproteobacterium isolated from soil. Int. J. Syst. Evol. Microbiol. 59: 1965-1968 [Abstract] [Full Text]  
  • Gueune, H., Durand, M.-J., Thouand, G., DuBow, M. S. (2008). The ygaVP Genes of Escherichia coli Form a Tributyltin-Inducible Operon. Appl. Environ. Microbiol. 74: 1954-1958 [Abstract] [Full Text]  
  • Sauve, V., Bruno, S., Berks, B. C., Hemmings, A. M. (2007). The SoxYZ Complex Carries Sulfur Cycle Intermediates on a Peptide Swinging Arm. J. Biol. Chem. 282: 23194-23204 [Abstract] [Full Text]  
  • Mandal, S., Chatterjee, S., Dam, B., Roy, P., Gupta, S. K. D. (2007). The dimeric repressor SoxR binds cooperatively to the promoter(s) regulating expression of the sulfur oxidation (sox) operon of Pseudaminobacter salicylatoxidans KCT001. Microbiology 153: 80-91 [Abstract] [Full Text]  
  • Wubbeler, J. H., Lutke-Eversloh, T., Van Trappen, S., Vandamme, P., Steinbuchel, A. (2006). Tetrathiobacter mimigardefordensis sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3'-dithiodipropionic acid. Int. J. Syst. Evol. Microbiol. 56: 1305-1310 [Abstract] [Full Text]  
  • Ghosh, W., Roy, P. (2006). Mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant. Int. J. Syst. Evol. Microbiol. 56: 91-97 [Abstract] [Full Text]  
  • Ghosh, W., Bagchi, A., Mandal, S., Dam, B., Roy, P. (2005). Tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in Jammu and Kashmir, India. Int. J. Syst. Evol. Microbiol. 55: 1779-1787 [Abstract] [Full Text]  
  • Appia-Ayme, C., Little, P. J., Matsumoto, Y., Leech, A. P., Berks, B. C. (2001). Cytochrome Complex Essential for Photosynthetic Oxidation of both Thiosulfate and Sulfide in Rhodovulum sulfidophilum. J. Bacteriol. 183: 6107-6118 [Abstract] [Full Text]  
  • Rother, D., Henrich, H.-J., Quentmeier, A., Bardischewsky, F., Friedrich, C. G. (2001). Novel Genes of the sox Gene Cluster, Mutagenesis of the Flavoprotein SoxF, and Evidence for a General Sulfur-Oxidizing System in Paracoccus pantotrophus GB17. J. Bacteriol. 183: 4499-4508 [Abstract] [Full Text]  
  • Friedrich, C. G., Rother, D., Bardischewsky, F., Quentmeier, A., Fischer, J. (2001). Oxidation of Reduced Inorganic Sulfur Compounds by Bacteria: Emergence of a Common Mechanism?. Appl. Environ. Microbiol. 67: 2873-2882 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»