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 Previous Article

Journal of Bacteriology, August 2000, p. 4372-4374, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of the Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase Gene

Ryan A. Mehl, Cynthia Kinsland, and Tadhg P. Begley*

Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York

Received 3 April 2000/Accepted 16 May 2000

The gene (ybeN) coding for nicotinate mononucleotide adenylyltransferase, an NAD(P) biosynthetic enzyme, has been identified and overexpressed in Escherichia coli. This enzyme catalyzes the reversible adenylation of nicotinate mononucleotide and shows product inhibition. The rate of adenylation of nicotinate mononucleotide is at least 20 times faster than the rate of adenylation of nicotinamide mononucleotide.

* Corresponding author. Mailing address: Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853. Phone: (607) 255-7133. Fax: (607) 255-4137. E-mail: tpb2{at}cornell.edu.

Journal of Bacteriology, August 2000, p. 4372-4374, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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