This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moch, C.
Right arrow Articles by Allmansberger, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moch, C.
Right arrow Articles by Allmansberger, R.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2000, p. 4384-4393, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Transcription of the nfrA-ywcH Operon from Bacillus subtilis Is Specifically Induced in Response to Heat

Christine Moch, Oliver Schrögel, and Rudolf Allmansberger*

Lehrstuhl für Mikrobiologie, Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany

Received 3 February 2000/Accepted 17 May 2000

The NfrA protein, an oxidoreductase from the soil bacterium Bacillus subtilis, is synthesized during the stationary phase and in response to heat. Analysis of promoter mutants revealed that the nfrA gene belongs to the class III heat shock genes in B. subtilis. An approximate 10-fold induction at both the transcriptional and the translational levels was found after thermal upshock. This induction resulted from enhanced synthesis of mRNA. Genetic and Northern blot analyses revealed that nfrA and the gene downstream of nfrA are transcribed as a bicistronic transcriptional unit. The unstable full-length transcript is processed into two short transcripts encoding nfrA and ywcH. The nfrA-ywcH operon is not induced by salt stress or by ethanol. According to previously published data, the transcription of class III genes in general is activated in response to the addition of these stressors. However, this conclusion is based on experiments which lacked a valid control. Therefore, it seems possible that the transcription of all class III genes is specifically induced by heat shock.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Universität Erlangen-Nürnberg, Staudstr. 5, D-91058 Erlangen, Germany. Phone: 49 9131 8528084. Fax: 49 9131 8528082. E-mail: rallmans{at}biologie.uni-erlangen.de.

Journal of Bacteriology, August 2000, p. 4384-4393, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Leelakriangsak, M., Kobayashi, K., Zuber, P. (2007). Dual Negative Control of spx Transcription Initiation from the P3 Promoter by Repressors PerR and YodB in Bacillus subtilis. J. Bacteriol. 189: 1736-1744 [Abstract] [Full Text]  
  • Streker, K., Freiberg, C., Labischinski, H., Hacker, J., Ohlsen, K. (2005). Staphylococcus aureus NfrA (SA0367) Is a Flavin Mononucleotide-Dependent NADPH Oxidase Involved in Oxidative Stress Response. J. Bacteriol. 187: 2249-2256 [Abstract] [Full Text]  
  • Movahedi, S., Waites, W. (2002). Cold Shock Response in Sporulating Bacillus subtilis and Its Effect on Spore Heat Resistance. J. Bacteriol. 184: 5275-5281 [Abstract] [Full Text]  
  • Helmann, J. D., Wu, M. F. W., Kobel, P. A., Gamo, F.-J., Wilson, M., Morshedi, M. M., Navre, M., Paddon, C. (2001). Global Transcriptional Response of Bacillus subtilis to Heat Shock. J. Bacteriol. 183: 7318-7328 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»