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Journal of Bacteriology, August 2000, p. 4587-4595, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Escherichia coli DNA Polymerase IV Mutator Activity: Genetic Requirements and Mutational Specificity

Jérôme Wagnerdagger and Takehiko Nohmi*

Division of Genetics and Mutagenesis, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan

Received 1 March 2000/Accepted 18 May 2000

The dinB gene of Escherichia coli is known to be involved in the untargeted mutagenesis of lambda  phage. Recently, we have demonstrated that this damage-inducible and SOS-controlled gene encodes a novel DNA polymerase, DNA Pol IV, which is able to dramatically increase the untargeted mutagenesis of F' plasmid. At the amino acid level, DNA Pol IV shares sequence homologies with E. coli UmuC (DNA Pol V), Rev1p, and Rad30p (DNA polymerase eta ) of Saccharomyces cerevisiae and human Rad30A (XPV) proteins, all of which are involved in translesion DNA synthesis. To better characterize the Pol IV-dependent untargeted mutagenesis, i.e., the DNA Pol IV mutator activity, we analyzed the genetic requirements of this activity and determined the forward mutation spectrum generated by this protein within the cII gene of lambda  phage. The results indicated that the DNA Pol IV mutator activity is independent of polA, polB, recA, umuDC, uvrA, and mutS functions. The analysis of more than 300 independent mutations obtained in the wild-type or mutS background revealed that the mutator activity clearly promotes single-nucleotide substitutions as well as one-base deletions in the ratio of about 1:2. The base changes were strikingly biased for substitutions toward G:C base pairs, and about 70% of them occurred in 5'-GX-3' sequences, where X represents the base (T, A, or C) that is mutated to G. These results are discussed with respect to the recently described biochemical characteristics of DNA Pol IV.

* Corresponding author. Mailing address: Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. Phone: 81 3 3700 9873. Fax: 81 3 3707 6950. E-mail: nohmi{at}nihs.go.jp.

dagger Present address: Institut de Recherche contre les Cancers de l'Appareil Digestif, Unite Propre de Recherche 9003 du Centre National de la Recherche Scientifique, Hôpitaux Universitaires, 67091 Strasbourg Cedex, France.

Journal of Bacteriology, August 2000, p. 4587-4595, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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