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Journal of Bacteriology, August 2000, p. 4632-4636, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Demonstration of a Novel Glycolytic Pathway in the Hyperthermophilic Archaeon Thermococcus zilligii by 13C-Labeling Experiments and Nuclear Magnetic Resonance Analysis

Karina B. Xavier,1 Milton S. da Costa,2 and Helena Santos1,*

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-156 Oeiras,1 and Departamento de Bioquímica and Centro de Neurociências de Coimbra, Universidade de Coimbra, 3000 Coimbra,2 Portugal

Received 3 March 2000/Accepted 26 May 2000

The operation of a novel glycolytic pathway was demonstrated in nongrowing cells of Thermococcus zilligii by analysis of the isotopic enrichment in the end products derived from fermentation of 13C-labeled glucose. The new pathway involved the formation of formate, derived from C-1 in glucose, via cleavage of a six-carbon carboxylic acid.

* Corresponding author. Mailing address: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2780-156 Oeiras, Portugal. Phone: 351-214469800. Fax: 351-214428766. E-mail: santos{at}itqb.unl.pt.

Journal of Bacteriology, August 2000, p. 4632-4636, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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