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Journal of Bacteriology, September 2000, p. 4979-4986, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Developmental Control of Stress Stimulons in Streptomyces coelicolor Revealed by Statistical Analyses of Global Gene Expression Patterns

J. Vohradsky,1,2,* X.-M. Li,1,dagger G. Dale,1,Dagger M. Folcher,1 L. Nguyen,1 P. H. Viollier,1 and C. J. Thompson1

Biozentrum, University of Basel, CH-4056 Basel, Switzerland,1 and Institute of Microbiology, Prague, Czech Republic2

Received 2 March 2000/Accepted 22 May 2000

Stress-induced regulatory networks coordinated with a procaryotic developmental program were revealed by two-dimensional gel analyses of global gene expression. Four developmental stages were identified by their distinctive protein synthesis patterns using principal component analysis. Statistical analyses focused on five stress stimulons (induced by heat, cold, salt, ethanol, or antibiotic shock) and their synthesis during development. Unlike other bacteria, for which various stresses induce expression of similar sets of protein spots, in Streptomyces coelicolor heat, salt, and ethanol stimulons were composed of independent sets of proteins. This suggested independent control by different physiological stress signals and their corresponding regulatory systems. These stress proteins were also under developmental control. Cluster analysis of stress protein synthesis profiles identified 10 different developmental patterns or "synexpression groups." Proteins induced by cold, heat, or salt shock were enriched in three developmental synexpression groups. In addition, certain proteins belonging to the heat and salt shock stimulons were coregulated during development. Thus, stress regulatory systems controlling these stimulons were implicated as integral parts of the developmental program. This correlation suggested that thermal shock and salt shock stress response regulatory systems either allow the cell to adapt to stresses associated with development or directly control the developmental program.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland. Phone: 41-61-267-2116. Fax: 41-61-267-2118. E-mail: Charles-J.Thompson{at}unibas.ch.

dagger Present address: University of Pennsylvania, Philadelphia, PA 19104.

Dagger Present address: F. Hoffmann-LaRoche AG, CH-4002 Basel, Switzerland.

Journal of Bacteriology, September 2000, p. 4979-4986, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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