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Journal of Bacteriology, September 2000, p. 5211-5217, Vol. 182, No. 18
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The NosX and NirX Proteins of Paracoccus denitrificans Are Functional Homologues: Their Role in Maturation of Nitrous Oxide Reductase

Neil F. W. Saunders,1,dagger Jorrit J. Hornberg,1 Willem N. M. Reijnders,1 Hans V. Westerhoff,1 Simon de Vries,2 and Rob J. M. van Spanning1,*

Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV Amsterdam,1 and Department of Biotechnology, Delft University of Technology, Delft,2 The Netherlands, European Union

Received 3 April 2000/Accepted 15 June 2000

The nos (nitrous oxide reductase) operon of Paracoccus denitrificans contains a nosX gene homologous to those found in the nos operons of other denitrifiers. NosX is also homologous to NirX, which is so far unique to P. denitrificans. Single mutations of these genes did not result in any apparent phenotype, but a double nosX nirX mutant was unable to reduce nitrous oxide. Promoter-lacZ assays and immunoblotting against nitrous oxide reductase showed that the defect was not due to failure of expression of nosZ, the structural gene for nitrous oxide reductase. Electron paramagnetic resonance spectroscopy showed that nitrous oxide reductase in cells of the double mutant lacked the CuA center. A twin-arginine motif in both NosX and NirX suggests that the NosX proteins are exported to the periplasm via the TAT translocon.

* Corresponding author. Mailing address: Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands. Phone: 31 20 4447179. Fax: 31 20 4447229. E-mail: spanning{at}bio.vu.nl.

dagger Present address: School of Microbiology and Immunology, University of New South Wales, Sydney 2052, New South Wales, Australia.

Journal of Bacteriology, September 2000, p. 5211-5217, Vol. 182, No. 18
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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