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Journal of Bacteriology, October 2000, p. 5454-5461, Vol. 182, No. 19
Department of Biotechnology, Fukuyama
University, Fukuyama, Hiroshima 729-0292, Japan,1 and Génétique
Microbienne, Institut National de la Recherche Agronomique, Domaine de
Vilvert, 78352 Jouy-en-Josas Cedex, France2
Received 16 May 2000/Accepted 9 July 2000
The ytrABCDEF operon of Bacillus subtilis
was deduced to encode a putative ATP-binding cassette (ABC) transport
system. YtrB and YtrE could be the ABC subunits, and YtrC and YtrD are
highly hydrophobic and could form a channel through the cell membrane, while YtrF could be a periplasmic lipoprotein for substrate binding. Expression of the operon was examined in cells grown in a minimal medium. The results indicate that the expression was induced only early
in the stationary phase. The six ytr genes form a single operon, transcribed from a putative
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
An Operon for a Putative ATP-Binding Cassette
Transport System Involved in Acetoin Utilization of
Bacillus subtilis
A-dependent promoter
present upstream of ytrA. YtrA, which possesses a
helix-turn-helix motif of the GntR family, acts probably as a repressor
and regulates its own transcription. Inactivation of the operon led to
a decrease in maximum cell yield and less-efficient sporulation,
suggesting its involvement in the growth in stationary phase and
sporulation. It is known that B. subtilis produces acetoin as an external carbon storage compound and then reuses it later during
stationary phase and sporulation. When either the entire ytr operon or its last gene, ytrF, was
inactivated, the production of acetoin was not affected, but the reuse
of acetoin became less efficient. We suggest that the Ytr transport
system plays a role in acetoin utilization during stationary phase and sporulation.
*
Corresponding author. Mailing address: Department of
Biotechnology, Fukuyama University, 985 Sanzo, Higashimura-cho,
Fukuyama, Hiroshima 729-0292, Japan. Phone: 81 849 36 2111. Fax: 81 849 36 2459. E-mail:
kyoshida{at}bt.fubt.fukuyama-u.ac.jp.
Journal of Bacteriology, October 2000, p. 5454-5461, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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