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Journal of Bacteriology, October 2000, p. 5556-5562, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Analysis of Nucleoid Morphology during Germination and Outgrowth of Spores of Bacillus Species

Katerina Ragkousi,1 Ann E. Cowan,1,2 Margery A. Ross,1 and Peter Setlow1,*

Department of Biochemistry1 and Center for Biomedical Imaging Technology,2 University of Connecticut Health Center, Farmington, Connecticut 06032

Received 17 April 2000/Accepted 5 July 2000

After a few minutes of germination, nucleoids in the great majority of spores of Bacillus subtilis and Bacillus megaterium were ring shaped. The major spore DNA binding proteins, the alpha /beta -type small, acid-soluble proteins (SASP), colocalized to these nucleoid rings early in spore germination, as did the B. megaterium homolog of the major B. subtilis chromosomal protein HBsu. The percentage of ring-shaped nucleoids was decreased in germinated spores with lower levels of alpha /beta -type SASP. As spore outgrowth proceeded, the ring-shaped nucleoids disappeared and the nucleoid became more compact. This change took place after degradation of most of the spores' pool of major alpha /beta -type SASP and was delayed when alpha /beta -type SASP degradation was delayed. Later in spore outgrowth, the shape of the nucleoid reverted to the diffuse lobular shape seen in growing cells.

* Corresponding author. Mailing address: Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032. Phone: (860) 679-2607. Fax: (860) 679-3408. E-mail: setlow{at}sun.uchc.edu.

Journal of Bacteriology, October 2000, p. 5556-5562, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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